LOCUS       sequence-99533-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter of activating sequence derived from native gal 
            promoter coupled with core promoter derived from native Leucine 
            promoter. Spacer between core and activating sequence was used. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: UASgal-A9-Pleum
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    425..542
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(594..614)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(594..612)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(633..649)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(633..649)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(646..668)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    657..673
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(681..687,688..705,706..711))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    726..747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 catctaattc aaccaaaatt gggacaacac cagtgaataa ttcttcacct ttagacattt
       61 ttctagatag aatggtatat ccttgaaata tatatatata tattgctgaa atgtaaaagg
      121 taagaaaagt tagaaagtaa gacgattgct aaccacctat tggaaaaaac aataggtcct
      181 taaataatat tggtttaaac catgtaaatt tcctaattgg gtaagtacat gaagcttctg
      241 ttaatagatc aaaaatcatc gcttcgctga ttaattaccc cagaaataag gctaaaaaac
      301 taatcgcatt atcatcctat ggttgttaat ttgattcgtt aatttgaagg tttgtggggc
      361 caggttactg ccaatttttc ctcttcataa ccataaaagc tagtattgta gaatctttat
      421 tgttcggagc agtgcggcgc gaggcacatc tgcgtttcag gaacgcgacc ggtgaagacg
      481 aggacgcacg gaggagagtc ttccgtcgga gggctgtcgc ccgctcggcg gcttctaatc
      541 cgtactatta attaaggggc gcgccaagga tcccggagct ccagcttttg ttccctttag
      601 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      661 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      721 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      781 ggaa
//
