LOCUS       sequence-99534-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter comprised of derivatives from native promoters 
            galactose, cyc and leucine..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: UASGAL-CU1-Pleum
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    516..633
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(685..705)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(685..703)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(724..740)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(724..740)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(737..759)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    748..764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 caccatctaa ttcaaccaaa attgggacaa caccagtgaa taattcttca cctttagaca
       61 tttttctaga tagaatggta tatccttgaa atatatatat atatattgct gaaatgtaaa
      121 aggtaagaaa agttagaaag taagacgatt gctaaccacc tattggaaaa aacaataggt
      181 ccttaaataa tattggttta aactgcctgt atgtgtcagc actaaagttg cctggccatc
      241 cacgctatat atacacgcct ggcggatctg ctcgaggatt gcctacgcgt gggcttgatc
      301 caccaaccaa cgctcgccaa ataagcttct gttaatagat caaaaatcat cgcttcgctg
      361 attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa
      421 tttgattcgt taatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata
      481 accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat
      541 ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt cttccgtcgg
      601 agggctgtcg cccgctcggc ggcttctaat ccgtactatt aattaagggg cgcgccaagg
      661 atcccggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
      721 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      781 agccggaagc a
//
