LOCUS       sequence-99535-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter comprised of derivatives from native promoters 
            galactose, cyc and leucine..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: UASGAL-CU2-Pleum
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    567..684
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(736..756)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(736..754)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(775..791)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(775..791)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(788..810)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    799..815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 catctaattc aaccaaaatt gggacaacac cagtgaataa ttcttcacct ttagacattt
       61 ttctagatag aatggtatat ccttgaaata tatatatata tattgctgaa atgtaaaagg
      121 taagaaaagt tagaaagtaa gacgattgct aaccacctat tggaaaaaac aataggtcct
      181 taaataatat tggtttaaac catacagagc acatgcatgc catatgatca tgtgtcgtcg
      241 cacacatata tatatgcctg tatgtgtcag cactaaagtt gcctggccat ccacgctata
      301 tatacacgcc tggcggatct gctcgaggat tgcctacgcg tgggcttgat ccaccaacca
      361 acgctcgcca aataagcttc tgttaataga tcaaaaatca tcgcttcgct gattaattac
      421 cccagaaata aggctaaaaa actaatcgca ttatcatcct atggttgtta atttgattcg
      481 ttaatttgaa ggtttgtggg gccaggttac tgccaatttt tcctcttcat aaccataaaa
      541 gctagtattg tagaatcttt attgttcgga gcagtgcggc gcgaggcaca tctgcgtttc
      601 aggaacgcga ccggtgaaga cgaggacgca cggaggagag tcttccgtcg gagggctgtc
      661 gcccgctcgg cggcttctaa tccgtactat taattaaggg gcgcgccaag gatcccggag
      721 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
      781 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagcc
//
