LOCUS       sequence-99537-e         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter of three gal binding sites derived from native 
            galactose promoter coupled with a core promoter derived from native 
            Leucine promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: Gal4pBS134-Pleum
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     192..209
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    326..347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(362..367,368..385,386..392)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    400..416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     405..427
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     424..440
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     424..440
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     459..479
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        461..479
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
       61 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      121 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      181 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
      241 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
      301 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
      361 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      421 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
      481 aacaaaagct ggagctccgg gatccttggc gcgcccctta attaacggaa gcttcggatt
      541 agaagccgcc gagcgggcga cagccctccg acggaagact ctcctccggt ttaaaccaat
      601 attatttaag gacctattgt ttt
//
