LOCUS       sequence-99538-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a promoter of three gal binding sites derived from native 
            galactose promoter coupled with a core promoter derived from native 
            Leucine promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Blazeck J, Garg R, Reed B, Alper HS
  TITLE     Controlling promoter strength and regulation in Saccharomyces 
            cerevisiae using synthetic hybrid promoters.
  JOURNAL   Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552.
            Epub 2012 May 17.
  PUBMED    22565375
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24552";
            journalName: "Biotechnol Bioeng"; date: "2012-11"; volume: "109"; 
            issue: "11"; pages: "2884-95"
COMMENT     Sequence Label: Gal4pBS134-Pleum
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(315..335)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(315..333)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(354..370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(354..370)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(367..389)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    378..394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(402..408,409..426,427..432))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    447..468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(585..602)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atcaccatct aattcaacca aaattgggac aacaccagtg aataattctt cacctttaga
       61 catttttcta gatagaatgg tatatccttg aaatatatat atatatattg ctgaaatgta
      121 aaaggtaaga aaagttagaa agtaagacga ttgctaacca cctattggaa aaaacaatag
      181 gtccttaaat aatattggtt taaaccggag gagagtcttc cgtcggaggg ctgtcgcccg
      241 ctcggcggct tctaatccga agcttccgtt aattaagggg cgcgccaagg atcccggagc
      301 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
      361 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      421 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      481 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      541 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      721 gccaggaacc gtaa
//
