LOCUS       sequence-99541-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dsRedExpress2 in response to doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Kabadi AM, Thakore PI, Vockely CM, Ousterout DG, Gibson TM, Guilak 
            F, Reddy TE, Gersbach CA
  TITLE     Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by 
            Fusion to a Potent Transactivation Domain.
  JOURNAL   ACS Synth Biol. 2014 Dec 10.
  PUBMED    25494287
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Dec 10."
COMMENT     Sequence Label: LV-TRE-Empty-T2A-dsRedExpress2
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(53..71,72..94,95..113,114..136,137..155,156..178,
                     179..197,198..220,221..239,240..262,263..281,282..304,
                     305..323)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        356..394
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     391..415
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             545..598
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(779..799)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 ttacaaaaat tcaaaatttt atcgatcacg agactagcct cgagtttacc actccctatc
       61 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
      121 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
      181 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
      241 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      301 ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag
      361 gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg
      421 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
      481 cggccccgaa ttcgagctcg gtacccgggg atcctctagt cagctgacgc gtttggttat
      541 gcatgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat
      601 ggatagcact gagaacgtca tcaagccctt catgcgcttc aaggtgcaca tggagggctc
      661 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggcaagccct acgagggcac
      721 ccagaccgcc aagctgcagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
      781 gtccccccag ttccagtacg gctccaaggt gtacgtgaag caccccgccg acatccccga
      841 ctacaagaag ctgtcctt
//
