LOCUS       sequence-99542-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CibN-dCas9-CibN in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     A light-inducible CRISPR-Cas9 system for control of endogenous gene 
            activation.
  JOURNAL   Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
  PUBMED    25664691
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753."
COMMENT     Sequence Label: pcDNA3.1-CibN-dCas9-CibN
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..541
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="NGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSSM
                     ITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCNE
                     KKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKEL
                     EKTDYI"
     CDS             548..571
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             578..598
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgacggtgat tataaagatc atgacatcga tataaatgga gctataggag gtgacctttt
       61 gctcaatttt cctgacatgt cggtcctaga gcgccaaagg gctcacctca agtacctcaa
      121 tcccaccttt gattctcctc tcgccggctt ctttgccgat tcttcaatga ttaccggcgg
      181 cgagatggac agctatcttt cgactgccgg tttgaatctt ccgatgatgt acggtgagac
      241 gacggtggaa ggtgattcaa gactctcaat ttcgccggaa acgacgcttg ggactggaaa
      301 tttcaagaaa cggaagtttg atacagagac taaggattgt aatgagaaga agaagaagat
      361 gacgatgaac agagatgacc tagtagaaga aggagaagaa gagaagtcga aaataacaga
      421 gcaaaacaat gggagcacaa aaagcatcaa gaagatgaaa cacaaagcca agaaagaaga
      481 gaacaatttc tctaatgatt catctaaagt gacgaaggaa ttggagaaaa cggattatat
      541 tcatcacgat tacaaggatg acgatgacaa gatggccccc aagaagaaga ggaaggtggg
      601 ccgcggaatg gataagaaat actcaatagg cttagctatc ggcacaaata gcgtcggatg
      661 ggcggtgatc actgatgaat ataaggttcc gt
//
