LOCUS       sequence-99544-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 
            6475312 (Invitrogen) and cloned downstream from the IRES; 
            subsequently Luciferase was cloned upstream of the IRES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Zuber J, Radtke I, Pardee TS, Zhao Z, Rappaport AR, Luo W, McCurrach
            ME, Yang MM, Dolan ME, Kogan SC, Downing JR, Lowe SW
  TITLE     Mouse models of human AML accurately predict chemotherapy response.
  JOURNAL   Genes Dev. 2009 Apr 1;23(7):877-89. doi: 10.1101/gad.1771409.
  PUBMED    19339691
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2009-04-1- 1"; volume: "23"; issue:
            "7"; pages: "877-89"
COMMENT     Sequence Label: MSCV-Luciferase-IRES-NrasG12D
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    189..756
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    206..758
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(350..367)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     577..596
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa
       61 tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa ttctaggatc
      121 cgtcgacctg cagccatgca ttgattcaaa attttccgat cacgagacta gcctcgaggt
      181 taacgaattc cgcccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
      241 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
      301 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
      361 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
      421 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
      481 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
      541 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
      601 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
      661 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
      721 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tgactgagta
      781 caaactggtg gtggttggag cagatggtgt tgggaaaagc gccttgacga tccagctaat
      841 ccagaaccac tttgtggatg aatatgatcc caccatagag gattcttacc gaaagcaagt
      901 ggtgattgat ggtgagacct gcctgctgga catactggac acagctggac aagaggagta
      961 cagtgcc
//
