LOCUS       sequence-99560-e         441 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in 
            neurons under control of truncated mecp2 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur
            M, Zhang F
  TITLE     In vivo interrogation of gene function in the mammalian brain using 
            CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.
  PUBMED    25326897
REFERENCE   2  (bases 1 to 441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055."
COMMENT     Sequence Label: PX551
FEATURES             Location/Qualifiers
     source          1..441
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             292..312
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    331..379
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc
       61 gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg
      121 tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac
      181 cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc
      241 accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgacag ccccaagaag
      301 aagagaaagg tggaggccag ctaagaattc aataaaagat ctttattttc attagatctg
      361 tgtgttggtt ttttgtgtgc ggccgcagga acccctagtg atggagttgg ccactccctc
      421 tctgcgcgct cgctcgctca c
//
