LOCUS       sequence-99561-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid
            for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells
            and nuclei. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur
            M, Zhang F
  TITLE     In vivo interrogation of gene function in the mammalian brain using 
            CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.
  PUBMED    25326897
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055."
COMMENT     Sequence Label: PX552
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     88..107
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        184..259
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        293..740
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      772..781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(823..844)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
       61 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
      121 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggaag agcgagctct
      181 tctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      241 agtggcaccg agtcggtgct ttttttctag actgcagagg gccctgcgta tgagtgcaag
      301 tgggttttag gaccaggatg aggcggggtg ggggtgccta cctgacgacc gaccccgacc
      361 cactggacaa gcacccaacc cccattcccc aaattgcgca tcccctatca gagaggggga
      421 ggggaaacag gatgcggcga ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc
      481 cttcgccccc gcctggcggc gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt
      541 cactcgccgg tcccccgcaa actccccttc ccggccacct tggtcgcgtc cgcgccgccg
      601 ccggcccagc cggaccgcac cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc
      661 tgcgctgcgg cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg
      721 tgtcgtgcct gagagcgcag tcgagaaggt accggatcct ctagagtcga cgccaccatg
      781 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      841 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      901 aagctgaccc tgaagttcat ctg
//
