LOCUS       sequence-99562-e         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid
            for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells
            and nuclei. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur
            M, Zhang F
  TITLE     In vivo interrogation of gene function in the mammalian brain using 
            CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.
  PUBMED    25326897
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055."
COMMENT     Sequence Label: PX552
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     384..405
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      447..456
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
       61 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      121 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      181 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca
      241 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      301 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      361 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
      421 ggtgaacagc tcctcgccct tgctcaccat ggtggcgtcg actctagagg atccggtacc
      481 ttctcgactg cgctctcagg cacgacacga ctcctccgct gcccaccgca gactgaggca
      541 gcgctgagtc gccggcgccg cagcgcagat ggtcgcgccc gtgcccccct atctcgcgcc
      601 tcgcgtggtg cggtccggct gggccggcgg cggcgcggac gcgaccaagg tggccgggaa
      661 ggggagtttg cgggggaccg gcgagtgacg tcagcgcgcc ttca
//
