LOCUS       sequence-99571-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid mediates polycistronic expression of 4 genes: Ngn3, 
            Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic 
            acinar cells to induced beta-like cells in vivo. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Li W, Cavelti-Weder C, Zhang Y, Clement K, Donovan S, Gonzalez G, 
            Zhu J, Stemann M, Xu K, Hashimoto T, Yamada T, Nakanishi M, Zhang Y,
            Zeng S, Gifford D, Meissner A, Weir G, Zhou Q
  TITLE     Long-term persistence and development of induced pancreatic beta 
            cells generated by lineage conversion of acinar cells.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1223-30. doi: 10.1038/nbt.3082. Epub
            2014 Nov 17.
  PUBMED    25402613
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3082"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1223-30"
COMMENT     Sequence Label: pAd-M3cherry
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      161..170
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agctatcaac aagtttgtac
      121 aaaaaagcag gctggcgccg gaaccaattc agtcgacgcc gccaccatgg cgcctcatcc
      181 cttggatgcg ctcaccatcc aagtgtcccc agagacacaa caaccttttc ccggagcctc
      241 ggaccacgaa gtgctcagtt ccaattccac cccacctagc cccactctca tacctaggga
      301 ctgctccgaa gcagaagtgg gtgactgccg agggacctcg aggaagctcc gcgcccgacg
      361 cggagggcgc aacaggccca agagcgagtt ggcactcagc aaacagcgaa gaagccggcg
      421 caagaaggcc aatgatcggg agcgcaatcg catgcacaac ctcaactcgg cgctggatgc
      481 gctgcgcggt gtcctgccca ccttcccgga tgacgccaaa cttacaaaga tcgagaccct
      541 gcgcttcgcc cacaactaca tctgggcact gactcagacg ctgcgcatag cggaccacag
      601 cttctatggc ccggagcccc ctgtgccctg tggagagctg gggagccccg gaggtggctc
      661 caacggggac tggggctcta tctactcccc agtctcccaa gcgggtaacc tgagccccac
      721 ggcctcattg gaggaattcc ctggcctgca ggtgcccagc tccccatcct atctgctccc
      781 gggagcactg gtgttctcag acttcttgac tagtgccacg aacttctctc tgttaaagca
      841 agcaggagat gttgaagaaa
//
