LOCUS       sequence-99572-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid mediates polycistronic expression of 4 genes: Ngn3, 
            Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic 
            acinar cells to induced beta-like cells in vivo. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Li W, Cavelti-Weder C, Zhang Y, Clement K, Donovan S, Gonzalez G, 
            Zhu J, Stemann M, Xu K, Hashimoto T, Yamada T, Nakanishi M, Zhang Y,
            Zeng S, Gifford D, Meissner A, Weir G, Zhou Q
  TITLE     Long-term persistence and development of induced pancreatic beta 
            cells generated by lineage conversion of acinar cells.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1223-30. doi: 10.1038/nbt.3082. Epub
            2014 Nov 17.
  PUBMED    25402613
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3082"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1223-30"
COMMENT     Sequence Label: pAd-M3cherry
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..57)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    83..107
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(131..841)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(409..428)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     678..696
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttactaaccg gtacgcgtag aatcgagacc gaggagaggg ttagggatag gcttaccttc
       61 gaaccgcggg ccctctagat caaccacttt gtacaagaaa gctgggtcta gatatctcga
      121 gtgcggccgc ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
      181 gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt
      241 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
      301 cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg
      361 cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca
      421 ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc
      481 cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca
      541 cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg
      601 ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga
      661 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
      721 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
      781 ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca
      841 ttgcggccgc
//
