LOCUS       sequence-99573-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid mediates polycistronic expression of 4 genes: Ngn3, 
            Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic 
            acinar cells to induced beta-like cells in vivo. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Li W, Cavelti-Weder C, Zhang Y, Clement K, Donovan S, Gonzalez G, 
            Zhu J, Stemann M, Xu K, Hashimoto T, Yamada T, Nakanishi M, Zhang Y,
            Zeng S, Gifford D, Meissner A, Weir G, Zhou Q
  TITLE     Long-term persistence and development of induced pancreatic beta 
            cells generated by lineage conversion of acinar cells.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1223-30. doi: 10.1038/nbt.3082. Epub
            2014 Nov 17.
  PUBMED    25402613
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3082"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1223-30"
COMMENT     Sequence Label: pAd-M3cherry
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(124..183)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             complement(649..678)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa
       61 gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca ccattgcggc
      121 cgcgggacct gggttgctct caacatctcc agccaatttc aagagagcat aattagtaca
      181 ctgatcgatc agaaagaagt cgggtgcgcc tttggccgcc ccggggccag cctgcgctgg
      241 cgagggctcc cgagggaagc cggccccgcc cgcgcccccg gggccgcccc ggcccgccaa
      301 cttctcgtat ttctccttgt acaggtcccg ctccttggcc agacgcccca cctccagctt
      361 cagctgctcc acctggctct ggagctggca cttctcgctc tccagaatgt gccgctgctg
      421 cacccgcttg aagcggcacg actgcgcgta gccgcggttc ttgagcgtgc gccgcttctg
      481 tttcagtcgg atgacctcct ccttgctgaa gccgcggagc tgccggttca gctcccgcac
      541 ggacatggat accagctggt cgtcggagaa gcgctcctcc aagcgcacgt ggtggcctgc
      601 gccgcctccg ccgtgacccg cgcctccgcc gtggtggcca gagcctccgt ggtggtgatg
      661 gtggtgatgg tgatggtggg cagagtgatg atggtggg
//
