LOCUS       sequence-99577-e        9390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide 
            RNA which target the NDM-1 gene. Contains a pBBR1 origin and 
            chloramphenicol resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Citorik RJ, Mimee M, Lu TK
  TITLE     Sequence-specific antimicrobials using efficiently delivered 
            RNA-guided nucleases.
  JOURNAL   Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.
  PUBMED    25240928
REFERENCE   2  (bases 1 to 9390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011."
COMMENT     Sequence Label: pMM178
FEATURES             Location/Qualifiers
     source          1..9390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      11..97
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    164..295
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   296..331
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   362..397
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     rep_origin      1859..2628
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             2629..3291
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     terminator      complement(3861..3955)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3976..4635)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4550..4569
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4636..4738)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4804..4822
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_RNA        complement(4908..4986)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     promoter        join(5151..5169,5170..5175,5176..5192,5193..5198,
                     5199..5224)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    5151..5169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5176..5194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             5234..5245
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5260..5277
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5278..9384
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5278..9381
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 ctagaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
       61 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctagcaccc
      121 tgcagaacta tatgattttc cgcagtatat tttagatgaa gattatttct taataactaa
      181 aaatatggta taatactctt aataaatgca gtaatacagg ggcttttcaa gactgaagtc
      241 tagctgagac aaatagtgcg attacgaaat tttttagaca aaaatagtct acgaggtttt
      301 agagctatgc tgttttgaat ggtcccaaaa cgggcagtcg cttccaacgg tttgatcgtc
      361 agttttagag ctatgctgtt ttgaatggtc ccaaaacttc agcacactga gacttgttga
      421 gttccatgtt ttagagctat gctgttttga atggtctcca ttctctagat gatgattaag
      481 gatctatttt tttgggcggg gccgcccaaa aaaatggttc gcttgctgtc cataaaaccg
      541 cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg
      601 cgttttccct tgtccagata gcccagtagc tgacattcat cccaggtggc acttttcggg
      661 gaaatgtgcg cgcccgcgtt cctgctggcg ctgggcctgt ttctggcgct ggacttcccg
      721 ctgttccgtc agcagctttt cgcccacggc cttgatgatc gcggcggcct tggcctgcat
      781 atcccgattc aacggcccca gggcgtccag aacgggcttc aggcgctccc gaaggtctcg
      841 ggccgtctct tgggcttgat cggccttctt gcgcatctca cgcgctcctg cggcggcctg
      901 tagggcaggc tcatacccct gccgaaccgc ttttgtcagc cggtcggcca cggcttccgg
      961 cgtctcaacg cgctttgaga ttcccagctt ttcggccaat ccctgcggtg cataggcgcg
     1021 tggctcgacc gcttgcgggc tgatggtgac gtggcccact ggtggccgct ccagggcctc
     1081 gtagaacgcc tgaatgcgcg tgtgacgtgc cttgctgccc tcgatgcccc gttgcagccc
     1141 tagatcggcc acagcggccg caaacgtggt ctggtcgcgg gtcatctgcg ctttgttgcc
     1201 gatgaactcc ttggccgaca gcctgccgtc ctgcgtcagc ggcaccacga acgcggtcat
     1261 gtgcgggctg gtttcgtcac ggtggatgct ggccgtcacg atgcgatccg ccccgtactt
     1321 gtccgccagc cacttgtgcg ccttctcgaa gaacgccgcc tgctgttctt ggctggccga
     1381 cttccaccat tccgggctgg ccgtcatgac gtactcgacc gccaacacag cgtccttgcg
     1441 ccgcttctct ggcagcaact cgcgcagtcg gcccatcgct tcatcggtgc tgctggccgc
     1501 ccagtgctcg ttctctggcg tcctgctggc gtcagcgttg ggcgtctcgc gctcgcggta
     1561 ggcgtgcttg agactggccg ccacgttgcc cattttcgcc agcttcttgc atcgcatgat
     1621 cgcgtatgcc gccatgcctg cccctccctt ttggtgtcca accggctcga cgggggcagc
     1681 gcaaggcggt gcctccggcg ggccactcaa tgcttgagta tactcactag actttgcttc
     1741 gcaaagtcgt gaccgcctac ggcggctgcg gcgccctacg ggcttgctct ccgggcttcg
     1801 ccctgcgcgg tcgctgcgct cccttgccag cccgtggata tgtggacgat ggccgcgagc
     1861 ggccaccggc tggctcgctt cgctcggccc gtggacaacc ctgctggaca agctgatgga
     1921 caggctgcgc ctgcccacga gcttgaccac agggattgcc caccggctac ccagccttcg
     1981 accacatacc caccggctcc aactgcgcgg cctgcggcct tgccccatca atttttttaa
     2041 ttttctctgg ggaaaagcct ccggcctgcg gcctgcgcgc ttcgcttgcc ggttggacac
     2101 caagtggaag gcgggtcaag gctcgcgcag cgaccgcgca gcggcttggc cttgacgcgc
     2161 ctggaacgac ccaagcctat gcgagtgggg gcagtcgaag gcgaagcccg cccgcctgcc
     2221 ccccgagcct cacggcggcg agtgcggggg ttccaagggg gcagcgccac cttgggcaag
     2281 gccgaaggcc gcgcagtcga tcaacaagcc ccggaggggc cactttttgc cggaggggga
     2341 gccgcgccga aggcgtgggg gaaccccgca ggggtgccct tctttgggca ccaaagaact
     2401 agatataggg cgaaatgcga aagacttaaa aatcaacaac ttaaaaaagg ggggtacgca
     2461 acagctcatt gcggcacccc ccgcaatagc tcattgcgta ggttaaagaa aatctgtaat
     2521 tgactgccac ttttacgcaa cgcataattg ttgtcgcgct gccgaaaagt tgcagctgat
     2581 tgcgcatggt gccgcaaccg tgcggcaccc taccgcatgg agataagcat ggccacgcag
     2641 tccagagaaa tcggcattca agccaagaac aagcccggtc actgggtgca aacggaacgc
     2701 aaagcgcatg aggcgtgggc cgggcttatt gcgaggaaac ccacggcggc aatgctgctg
     2761 catcacctcg tggcgcagat gggccaccag aacgccgtgg tggtcagcca gaagacactt
     2821 tccaagctca tcggacgttc tttgcggacg gtccaatacg cagtcaagga cttggtggcc
     2881 gagcgctgga tctccgtcgt gaagctcaac ggccccggca ccgtgtcggc ctacgtggtc
     2941 aatgaccgcg tggcgtgggg ccagccccgc gaccagttgc gcctgtcggt gttcagtgcc
     3001 gccgtggtgg ttgatcacga cgaccaggac gaatcgctgt tggggcatgg cgacctgcgc
     3061 cgcatcccga ccctgtatcc gggcgagcag caactaccga ccggccccgg cgaggagccg
     3121 cccagccagc ccggcattcc gggcatggaa ccagacctgc cagccttgac cgaaacggag
     3181 gaatgggaac ggcgcgggca gcagcgcctg ccgatgcccg atgagccgtg ttttctggac
     3241 gatggcgagc cgttggagcc gccgacacgg gtcacgctgc cgcgccggta gcacttgggt
     3301 tgcgcagcaa cccgtaagtg cgctgttcca gactatcggc tgtagccgcc tcgccgccct
     3361 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
     3421 aatggaagcc ggcggcacct cgctaacgga ttcaccgttt ttatcaggct ctgggaggca
     3481 gaataaatga tcatatcgtc aattattacc tccacgggga gagcctgagc aaactggcct
     3541 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
     3601 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg
     3661 ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag caccaggcgt
     3721 ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagtc
     3781 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3841 attaacgcac tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     3901 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     3961 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     4021 aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg
     4081 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     4141 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     4201 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     4261 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     4321 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     4381 ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag
     4441 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     4501 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     4561 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     4621 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     4681 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac
     4741 gtctcatttt cgccagatat cgacgtctaa gaaaccatta ttatcatgac attaacctat
     4801 aaaaataggc gtatcacgag gccctttcgt cttcaccctg tcgacgcgtt ttgaaagcgc
     4861 ttgttttttt ggtttgcagt cagagtagaa tagaagtatc aaaaaaagca ccgactcggt
     4921 gccacttttt caagttgata acggactagc cttattttaa cttgctatgc tgttttgaat
     4981 ggttccaaca agattatttt ataactttta taacaaataa tcaaggagaa attcaaagaa
     5041 atttatcagc cataaaacaa tacttaatac tatagaatga taacaaaata aactactttt
     5101 taaaagaatt ttgtgttata atctatttat tattaagtat tgggatcgat tccctatcag
     5161 tgatagagat tgacatccct atcagtgata gagatactga gcacatcagc aggacgcact
     5221 gaccgaattc attaaagagg agaaaggtgc ggccgcatgc atcaccatca ccatcacatg
     5281 gataagaaat actcaatagg cttagatatc ggcacaaata gcgtcggatg ggcggtgatc
     5341 actgatgaat ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac
     5401 agtatcaaaa aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg
     5461 actcgtctca aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat
     5521 ctacaggaga ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt
     5581 gaagagtctt ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat
     5641 atagtagatg aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa
     5701 ttggtagatt ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg
     5761 attaagtttc gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg
     5821 gacaaactat ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt
     5881 aacgcaagtg gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga
     5941 ttagaaaatc tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc
     6001 attgctttgt cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat
     6061 gctaaattac agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa
     6121 attggagatc aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta
     6181 ctttcagata tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg
     6241 attaaacgct acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa
     6301 caacttccag aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt
     6361 tatattgatg ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa
     6421 aaaatggatg gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag
     6481 caacggacct ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct
     6541 attttgagaa gacaagaaga cttttatcca tttttaaaag acaatcgtga gaagattgaa
     6601 aaaatcttga cttttcgaat tccttattat gttggtccat tggcgcgtgg caatagtcgt
     6661 tttgcatgga tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt
     6721 gtcgataaag gtgcttcagc tcaatcattt attgaacgca tgacaaactt tgataaaaat
     6781 cttccaaatg aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat
     6841 aacgaattga caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca
     6901 ggtgaacaga agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt
     6961 aagcaattaa aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca
     7021 ggagttgaag atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt
     7081 aaagataaag attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta
     7141 acattgacct tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac
     7201 ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggacgt
     7261 ttgtctcgaa aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat
     7321 tttttgaaat cagatggttt tgccaatcgc aattttatgc agctgatcca tgatgatagt
     7381 ttgacattta aagaagacat tcaaaaagca caagtgtctg gacaaggcga tagtttacat
     7441 gaacatattg caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta
     7501 aaagttgttg atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt
     7561 gaaatggcac gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg
     7621 aaacgaatcg aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt
     7681 gaaaatactc aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac
     7741 atgtatgtgg accaagaatt agatattaat cgtttaagtg attatgatgt cgatcacatt
     7801 gttccacaaa gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat
     7861 aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac
     7921 tattggagac aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg
     7981 aaagctgaac gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg
     8041 gttgaaactc gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact
     8101 aaatacgatg aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa
     8161 ttagtttctg acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac
     8221 catcatgccc atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat
     8281 ccaaaacttg aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg
     8341 attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat
     8401 atcatgaact tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct
     8461 ctaatcgaaa ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc
     8521 acagtgcgca aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag
     8581 acaggcggat tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct
     8641 cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat
     8701 tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa
     8761 gagttactag ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt
     8821 ttagaagcta aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat
     8881 agtctttttg agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa
     8941 aaaggaaatg agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat
     9001 tatgaaaagt tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag
     9061 cataagcatt atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt
     9121 ttagcagatg ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca
     9181 atacgtgaac aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc
     9241 gctgctttta aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa
     9301 gttttagatg ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat
     9361 ttgagtcagc taggaggtga ctgactcgag
//
