LOCUS       sequence-99583-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E
  TITLE     A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive 
            Bacterial Immunity.
  JOURNAL   Science. 2012 Aug 17;337(6096):816-21. doi: 10.1126/science.1225829.
            Epub 2012 Jun 28.
  PUBMED    22745249
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1225829"; journalName: "Science"; date: 
            "2012-08-17- 17"; volume: "337"; issue: "6096"; pages: "816-21"
COMMENT     Sequence Label: pMJ823
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag ttagtcatcc
       61 agcctttttc gtgattcata tagtcctgtg attgattggt agattatggt ggagcttaat
      121 agttctttta gattgttata tcttttacgc tcaatagtgg tttcaaaaaa tttaaaactt
      181 gccggagcac ccatcgcatt gaatgccatt aaatcaacaa aagatcgtgc aattgccttg
      241 atgtcacctt ctttattttg ctcaaaaagc tgattaattt tgtttagatt agcttcagct
      301 aacgtatatc ttttggcaaa ttctgatacg tgcgctagta gttcagcaaa catttctcta
      361 ttactttcaa tataatcaag gcttttccca tcacttgcct cacaatttgc cgcatggtgt
      421 agtaatgtta ccaggtggtt tggcaatacc tgctgatttc ctttctgagc ttcattagca
      481 ctagccagca ttctccgacg tccttcctca cattcatata acgtatattt cggtaatttc
      541 gcgaggactt taggctgacg ataaccttgt tcttctaaaa aggctttctc atctttttca
      601 aatgctttac gttccatgat agtaacacgg ataatttttt tctcgaaaac taatttattt
      661 ttcccttttg catattcaat aaccactgca tatgccatat tagggctatc aagaccacca
      721 tatttcatcg ggtcccaatt agtttttcta ggaattaatt tactggaatt tcctttgggc
      781 ttaattgtag cttttgaaaa ttctcctttt tgaatttctg ttttcttaac aatattcatt
      841 tgtcgataac tcatgacttt tttgacagta tccaaatatt ttttatccca aagaatttca
      901 ccgttttcat ctattattcg atctttttgc gcaaagaata acatga
//
