LOCUS       sequence-99598-e         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Expression Vector for Cas9 H840A nickase and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Miyaoka Y, Berman JR, Cooper SB, Mayerl SJ, Chan AH, Zhang B, 
            Karlin-Neumann GA, Conklin BR
  TITLE     Systematic quantification of HDR and NHEJ reveals effects of locus, 
            nuclease, and cell type on genome-editing.
  JOURNAL   Sci Rep. 2016 Mar 31;6:23549. doi: 10.1038/srep23549.
  PUBMED    27030102
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-03-31"; volume: "6:23549. doi"; pages: " 
            10.1038/srep23549"
COMMENT     Sequence Label: pXCas9H840A
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      195..204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             204..269
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             276..296
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
       61 gacggccctt ctcctccggg ctgtaattag ctgagcaaga ggtaagggtt taagggatgg
      121 ttggttggtg gggtattaat gtttaattac ctggagcacc tgcctgaaat cacttttttt
      181 caggttggac cggtgccacc atggactata aggaccacga cggagactac aaggatcatg
      241 atattgatta caaagacgat gacgataaga tggccccaaa gaagaagcgg aaggtcggta
      301 tccacggagt cccagcagcc gacaagaagt acagcatcgg cctggacatc ggcaccaact
      361 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
      421 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
      481 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
      541 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
      601 acagcttctt ccacagactg
//
