LOCUS       sequence-99599-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Expression Vector for Cas9 H840A nickase and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Miyaoka Y, Berman JR, Cooper SB, Mayerl SJ, Chan AH, Zhang B, 
            Karlin-Neumann GA, Conklin BR
  TITLE     Systematic quantification of HDR and NHEJ reveals effects of locus, 
            nuclease, and cell type on genome-editing.
  JOURNAL   Sci Rep. 2016 Mar 31;6:23549. doi: 10.1038/srep23549.
  PUBMED    27030102
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-03-31"; volume: "6:23549. doi"; pages: " 
            10.1038/srep23549"
COMMENT     Sequence Label: pXCas9H840A
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             661..708
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(736..753)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc
       61 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa
      121 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
      181 ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg
      241 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
      301 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
      361 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
      421 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
      481 atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac
      541 accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc
      601 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac
      661 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaagta agaattccta
      721 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
      781 cccccgtgcc ttccttgacc ctggaaggtg ccactccc
//
