LOCUS       sequence-99601-e         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Expression Vector for FokI-dCas9 and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Miyaoka Y, Berman JR, Cooper SB, Mayerl SJ, Chan AH, Zhang B, 
            Karlin-Neumann GA, Conklin BR
  TITLE     Systematic quantification of HDR and NHEJ reveals effects of locus, 
            nuclease, and cell type on genome-editing.
  JOURNAL   Sci Rep. 2016 Mar 31;6:23549. doi: 10.1038/srep23549.
  PUBMED    27030102
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-03-31"; volume: "6:23549. doi"; pages: " 
            10.1038/srep23549"
COMMENT     Sequence Label: pXFokI-dCas9
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        31..106
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        203..488
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ggacgaaaca ccgggtcttc gagaagacct gttttagagc tagaaatagc aagttaaaat
       61 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga
      121 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca
      181 gacaaatggc tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac
      241 cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac
      301 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      361 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc
      421 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      481 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
      541 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg g
//
