LOCUS       sequence-99602-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Expression Vector for FokI-dCas9 and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Miyaoka Y, Berman JR, Cooper SB, Mayerl SJ, Chan AH, Zhang B, 
            Karlin-Neumann GA, Conklin BR
  TITLE     Systematic quantification of HDR and NHEJ reveals effects of locus, 
            nuclease, and cell type on genome-editing.
  JOURNAL   Sci Rep. 2016 Mar 31;6:23549. doi: 10.1038/srep23549.
  PUBMED    27030102
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-03-31"; volume: "6:23549. doi"; pages: " 
            10.1038/srep23549"
COMMENT     Sequence Label: pXFokI-dCas9
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             620..640
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggggataacg attatggagc gctcgtcttt tgaaaagaac cccatcgact tccttgaggc
       61 gaaaggttac aaggaagtaa aaaaggatct cataattaaa ctaccaaagt atagtctgtt
      121 tgagttagaa aatggccgaa aacggatgtt ggctagcgcc ggagagcttc aaaaggggaa
      181 cgaactcgca ctaccgtcta aatacgtgaa tttcctgtat ttagcgtccc attacgagaa
      241 gttgaaaggt tcacctgaag ataacgaaca gaagcaactt tttgttgagc agcacaaaca
      301 ttatctcgac gaaatcatag agcaaatttc ggaattcagt aagagagtca tcctagctga
      361 tgccaatctg gacaaagtat taagcgcata caacaagcac agggataaac ccatacgtga
      421 gcaggcggaa aatattatcc atttgtttac tcttaccaac ctcggcgctc cagccgcatt
      481 caagtatttt gacacaacga tagatcgcaa acgatacact tctaccaagg aggtgctaga
      541 cgcgacactg attcaccaat ccatcacggg attatatgaa actcggatag atttgtcaca
      601 gcttgggggt gacggatccc ccaagaagaa gaggaaagtc tcgagcgact acaaagacca
      661 tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca a
//
