LOCUS       sequence-99605-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter construct for lineage tracing and mosaic analysis in 
            zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Collins RT, Linker C, Lewis J
  TITLE     MAZe: a tool for mosaic analysis of gene function in zebrafish.
  JOURNAL   Nat Methods. 2010 Mar;7(3):219-23. doi: 10.1038/nmeth.1423. Epub 
            2010 Feb 7.
  PUBMED    20139970
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1423"; journalName: "Nat Methods"; date: "2010-03"; 
            volume: "7"; issue: "3"; pages: "219-23"
COMMENT     Sequence Label: pMAZe
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    79..213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(103..122)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     157..176
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     339..355
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     339..355
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    377..471
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             join(568..576,577..591)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      598..607
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccgtccccgg ttcgtatcca caataaacac gagcacatac attacaaaaa cctgcggttg
       61 tcgtctgatt atttggtgga tccagacatg ataagataca ttgatgagtt tggacaaacc
      121 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
      181 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
      241 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
      301 ggtatggctg attatgatcc tctagagcgg ccggccgctc tagaactagt ggatccaagc
      361 ttgcatgcct gcaggtcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
      421 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagactc
      481 tagagggtat ataatggatc ccatcgcgtc tcagcctcac tttgagctcc tccacacgaa
      541 ttccccgcca cctaacaaat gggtcgggat ctgtacgacg atgacgataa ggatcccgcc
      601 accatggtga gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg
      661 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcga
//
