LOCUS       sequence-99606-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CaMPARI W391F V398L in mammalian cells, a photoconvertible
            fluorescent protein-based calcium integrator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Fosque BF, Sun Y, Dana H, Yang CT, Ohyama T, Tadross MR, Patel R, 
            Zlatic M, Kim DS, Ahrens MB, Jayaraman V, Looger LL, Schreiter ER
  TITLE     Neural circuits. Labeling of active neural circuits in vivo with 
            designed calcium integrators.
  JOURNAL   Science. 2015 Feb 13;347(6223):755-60. doi: 10.1126/science.1260922.
  PUBMED    25678659
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-02-13- 13"; volume: "347"; 
            issue: "6223"; pages: "755-60"
COMMENT     Sequence Label: pcDNA3-CaMPARI W391F V398L
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..518
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 acgagcttgc tcttaagttg gctggacttg atattaacaa gactggaggt tctcatcatc
       61 accaccacca tggatccgac caactgactg aagagcagat cgcagaattt aaagaggctt
      121 tctccctatt tgacaaggac ggggatggga caataacaac caaggagctg gggacggtga
      181 tgcggtctct ggggcagaac cccacagaag cagagctgca ggacatgatc aatgaagtag
      241 atgccgacgg tgacggcaca atcgacttcc ctgagttcct gacaatgatg gcaagaaaaa
      301 tgaaagacac agacagtgaa gaagaaatta gagaagcgtt ccgtgtgttt gataaggatg
      361 gcaatggcta catcagtgca gcagagcttc gccacgtgat gacaaacctt ggagagaagt
      421 taacagatga agaggttgat gaaatgatca gggaagcaga catcgatggg gatggtcagg
      481 taaactacga agagtttgta caaatgatga cagcgaagct cgagtgcgag aaaatctatg
      541 tgcgtgatgg agtgctgacg ggtgatattc atatgatctt gttgcttgaa ggaaatgccc
      601 attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc aagttaccag
      661 gcgtgcactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat tacaacaagg
      721 ttaagctgta tgagtatgct gttgctcatt ctggattgcc tgacaatgcc agacgaggcg
      781 gtaccggcgg atccatggtg agtgcgatta agccagacat gaagatcaaa ctccgtatgg
      841 aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttatg
      901 agggaaaaca gaccatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg
      961 atatcctgac cactgcattc cattacggc
//
