LOCUS       sequence-99614-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI D-E 11GFP
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     249..267
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(305..327)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     427..446
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     640..662
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     654..671
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     655..671
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             708..731
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             759..806
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg
       61 caagcagatt acggtgacga tcccgcagtg gctctctata caaagttggg catacgggaa
      121 gaagtgatgc actttgatat cgacccaagt accgccacct aacaattcgt tcaagccgag
      181 atcggcttcc cggccgcgga gttgttcggt aaattgtcac aacgccgctc atgacattaa
      241 cctataaaaa taggcgtatc acgaggccct tttgtctcgc gcgtttcggt gatgacggtg
      301 aaaacctctg acacatgcag ctcccggaga aggtcacagc ttgtctgtaa gcggatgccg
      361 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
      421 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
      481 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
      541 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
      601 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
      661 cgacggccag tgaattcgag ctcggtacct cgatggtctc aaagggagac tacaaggacg
      721 acgatgacaa gggcgacggc ggcagcggcg gcggcagcag ggaccacatg gttctccacg
      781 aatacgtcaa cgccgccgga atcacataaa atcagagacc tacgaatgca tctagatatc
      841 ggatcccggg cccgtcgact gcagagg
//
