LOCUS       sequence-99616-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI C-D 11GFP
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..110)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(138..161)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(199..216)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(199..215)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(208..230)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(424..443)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     543..565
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(603..621)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcctctgcag tcgacgggcc cgggatccga tatctagatg cattcgtagg tctctccttt
       61 tatgtgattc cggcggcgtt gacgtattcg tggagaacca tgtggtccct gctgccgccg
      121 ccgctgccgc cgtcgccctt gtcatcgtcg tccttgtagt ccatggtgtg agaccatcga
      181 ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      241 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      301 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
      361 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
      421 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
      481 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgacctt
      541 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacaaaa
      601 gggcctcgtg atacgcctat ttttataggt taatgtcatg agcggcgttg tgacaattta
      661 ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta
      721 cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc
      781 actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg
      841 gcctcatgc
//
