LOCUS       sequence-99617-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI D-E 1-10GFP
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(783..799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(783..799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(796..818)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    807..823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(831..837,838..855,856..861))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    876..897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gatatcggat cccgggcccg tcgactgcag aggtacgggt ctcaaaggga ggtggaggag
       61 gttctggagg cggtggaagt ggtggcggag gtagcgtgtc taagggcgaa gaactcttca
      121 ctggtgttgt gcctatcctc gtggaactcg acggcgacgt taacggtcat aagttctccg
      181 tcaggggaga gggagaaggc gacgctacca tcggaaagct cacactcaag ttcatctgca
      241 ccacaggcaa gctccctgtg ccttggccta ctctcgttac taccctcacc tacggtgttc
      301 agtgcttctc tcgttaccct gaccacatga agcagcatga cttcttcaag agtgctatgc
      361 ctgagggcta cgtccaggaa cgtacaatct ctttcaagga cgacggaaag tacaagacaa
      421 gggccgtcgt gaagttcgag ggtgacactc tcgtgaaccg tatcgaactc aagggaaccg
      481 acttcaagga ggacggtaac atcctcggtc acaagctcga atacaacttc aactcccata
      541 acgtgtacat cacagctgac aagcagaaga acggaatcaa ggccaacttc actgttaggc
      601 acaacgtcga ggacggttct gttcagctcg ccgaccatta ccagcagaac acccctatcg
      661 gtgacggacc tgtcctcctc cctgacaacc actacctctc tacacagact gtgctctcca
      721 aggaccctaa cgaaaagtaa aatctgagac ctctgcctgc atgcaagctt ggcgtaatca
      781 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      841 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      901 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      961 atcgg
//
