LOCUS       sequence-99620-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI C-D 1-10GFP
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(726..742)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(726..742)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(739..761)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    750..766
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(774..780,781..798,799..804))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    819..840
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgttagggtc cttggagagc acagtctgtg tagagaggta gtggttgtca gggaggagga
       61 caggtccgtc accgataggg gtgttctgct ggtaatggtc ggcgagctga acagaaccgt
      121 cctcgacgtt gtgcctaaca gtgaagttgg ccttgattcc gttcttctgc ttgtcagctg
      181 tgatgtacac gttatgggag ttgaagttgt attcgagctt gtgaccgagg atgttaccgt
      241 cctccttgaa gtcggttccc ttgagttcga tacggttcac gagagtgtca ccctcgaact
      301 tcacgacggc ccttgtcttg tactttccgt cgtccttgaa agagattgta cgttcctgga
      361 cgtagccctc aggcatagca ctcttgaaga agtcatgctg cttcatgtgg tcagggtaac
      421 gagagaagca ctgaacaccg taggtgaggg tagtaacgag agtaggccaa ggcacaggga
      481 gcttgcctgt ggtgcagatg aacttgagtg tgagctttcc gatggtagcg tcgccttctc
      541 cctctcccct gacggagaac ttatgaccgt taacgtcgcc gtcgagttcc acgaggatag
      601 gcacaacacc agtgaagagt tcttcgccct tagacaccat ggtgtgagac catcgaatgc
      661 atctagatat cggatcccgg gcccgtcgac tgcagaggcc tgcatgcaag cttggcgtaa
      721 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      781 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      841 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      901 tgaatcggcc aa
//
