LOCUS       sequence-99622-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI C-D 1-10GFP noATG
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(723..739)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(723..739)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(736..758)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    747..763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(771..777,778..795,796..801))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    816..837
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gggcgaagaa ctcttcactg gtgttgtgcc tatcctcgtg gaactcgacg gcgacgttaa
       61 cggtcataag ttctccgtca ggggagaggg agaaggcgac gctaccatcg gaaagctcac
      121 actcaagttc atctgcacca caggcaagct ccctgtgcct tggcctactc tcgttactac
      181 cctcacctac ggtgttcagt gcttctctcg ttaccctgac cacatgaagc agcatgactt
      241 cttcaagagt gctatgcctg agggctacgt ccaggaacgt acaatctctt tcaaggacga
      301 cggaaagtac aagacaaggg ccgtcgtgaa gttcgagggt gacactctcg tgaaccgtat
      361 cgaactcaag ggaaccgact tcaaggagga cggtaacatc ctcggtcaca agctcgaata
      421 caacttcaac tcccataacg tgtacatcac agctgacaag cagaagaacg gaatcaaggc
      481 caacttcact gttaggcaca acgtcgagga cggttctgtt cagctcgccg accattacca
      541 gcagaacacc cctatcggtg acggacctgt cctcctccct gacaaccact acctctctac
      601 acagactgtg ctctccaagg accctaacga aaagtgaaag gagagaccta cgaatgcatc
      661 tagatatcgg atcccgggcc cgtcgactgc agaggcctgc atgcaagctt ggcgtaatca
      721 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      781 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      841 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      901 atcggccaac gcgc
//
