LOCUS       sequence-99625-e         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIalpha F E-F
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             201..506
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     385..404
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
       61 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
      121 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
      181 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
      241 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
      301 tccccctggc cagtgcacgt ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg
      361 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggttt
      421 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
      481 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag
      541 gtcgacgaag actttctgtg agtgagacct
//
