LOCUS       sequence-99629-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Kir2.1-T2A-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Xue M, Atallah BV, Scanziani M
  TITLE     Equalizing excitation-inhibition ratios across visual cortical 
            neurons.
  JOURNAL   Nature. 2014 Jul 31;511(7511):596-600. doi: 10.1038/nature13321. 
            Epub 2014 Jun 22.
  PUBMED    25043046
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13321"; journalName: "Nature"; date: "2014-07-31- 
            31"; volume: "511"; issue: "7511"; pages: "596-600"
COMMENT     Sequence Label: pCAG-Kir2.1-T2A-tdTomato
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(92..115)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(290..309)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     559..577
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      707..716
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctgccgctgc cggtgctgcc ggtgccatgc cccaggaaca ggtggtggcg gccctcggag
       61 cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc cagcttggtg
      121 tccacgtagt agtagccggg cagttgcacg ggcttcttgg ccatgtagat ggtcttgaac
      181 tccaccaggt agtggccgcc gtccttcagc ttcagggcct ggtggatctc gcccttcagc
      241 acgccgtcgc gggggtacag gcgctcggtg gaggcctccc agcccatggt cttcttctgc
      301 attacggggc cgtcgggggg gaagttggtg ccgcgcatct tcaccttgta gatcagcgtg
      361 ccgtcctgca gggaggagtc ctgggtcacg gtcaccagac cgccgtcctc gaagttcatc
      421 acgcgctccc acttgaagcc ctcggggaag gacagcttct tgtaatcggg gatgtcggcg
      481 gggtgcttca cgtacgcctt ggagccgtac atgaactggg gggacaggat gtcccaggcg
      541 aagggcaggg ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
      601 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcatggagcc ctccatgcgc
      661 accttgaagc gcatgaactc tttgatgacc tcctcgccct tgctcaccat ggtggcggga
      721 tcc
//
