LOCUS       sequence-99631-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flp-dependent expression of Kir2.1-T2A-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Xue M, Atallah BV, Scanziani M
  TITLE     Equalizing excitation-inhibition ratios across visual cortical 
            neurons.
  JOURNAL   Nature. 2014 Jul 31;511(7511):596-600. doi: 10.1038/nature13321. 
            Epub 2014 Jun 22.
  PUBMED    25043046
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13321"; journalName: "Nature"; date: "2014-07-31- 
            31"; volume: "511"; issue: "7511"; pages: "596-600"
COMMENT     Sequence Label: pAAV-EF1-alpha-F-FLEX- Kir2.1-T2A-tdTomato
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..82
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      211..220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             220..249
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattctgga agttcctatt
       61 ctctagaaag tataggaact tctctttgca ccattctaaa gaataacagt gataatttct
      121 gggttaaggc aagaagttcc tattctatca gaagtatagg aacttcttaa ttaaggcgcg
      181 cctacgtagc tagccgggat ccgcggccgc gccaccatgg agcagaagct gatcagcgag
      241 gaggacctgg gcagtgtgag aaccaaccgg tacagcattg tctcttcgga ggaagatggc
      301 atgaagctgg ccactatggc agttgccaat ggctttggga atggcaagag taaagtccat
      361 acccgacaac agtgcaggag ccgctttgtg aagaaagatg gtcattgcaa tgttcagttt
      421 atcaacgtgg gtgagaaggg acagaggtac ctggcagaca tctttactac ctgtgtcgac
      481 atccgctgga ggtggatgct ggttatcttc tgtcttgcct tcgtgctctc ctggctgttc
      541 tttggctgtg tgttttggtt gatagccctg ctccatgggg atctagatac ttctaaagtg
      601 agcaaagcat gcgtgtcgga ggttaacagc ttcacggctg ccttcctctt ctccatcgag
      661 acccagacaa ccattggcta tggtttcagg tgtgtgacag acgaatgccc aattgctgtc
      721 ttcatggtgg tattccagtc aattgtaggc tgcatcattg acgccttcat cattggtgca
      781 gtcatggcga
//
