LOCUS       sequence-99634-e       10899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vanillate-inducible gene expression plasmid for Sphingomonas melonis
            Fr1; destination vector for Gateway cloning, allowing C-terminal 
            fusion of proteins to SYFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Synthetic vanillate-regulated promoter for graded gene expression in
            Sphingomonas.
  JOURNAL   Sci Rep. 2014 Sep 29;4:6453. doi: 10.1038/srep06453.
  PUBMED    25262659
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-09-29"; volume: "4:6453. doi"; pages: " 
            10.1038/srep06453"
COMMENT     Sequence Label: pVYD
FEATURES             Location/Qualifiers
     source          1..10899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..642
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             join(2636..2638,2639..2641,2642..3352)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2681..2702)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2942..2961)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3289..3310
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3371..3495
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3520..3525,3526..3543,3544..3550)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3604..4263
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3670..3689)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      4316..4904
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5058..5075
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             5537..5842
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5721..5740
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5883..6007)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6049..6114
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(6186..6203)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6186..6202)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6195..6217)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6411..6430)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6530..6552
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6590..6608)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             7394..8593
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(8824..9972)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10244..10615)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            10648..10757
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttcagtc ggcgcgaatg ctccacgccg cgcccagcgg cgcgccggcg
     1801 ctcgccgcgg cctcgaagac cttggcgtta cgaatggcgg ccagggcatg gtcgcgcatg
     1861 atccgctcgg ccccctcggc gtcgccgcac gagaccgcgt ccagcaccgc ctgatgctgg
     1921 cgatgggcgg ccagaaggtg ttcgtactcg gccggcaagt ccatcagatc cagggccagc
     1981 gcaccggccg ccgcgaacgg ctcgaaacca ttgcgcgcca gggcgctctc gacagcgccg
     2041 ttgcccgcag cgctgaccag cgtgtcatgg aaggcctgat tgtacgcggc gtagcgatcc
     2101 aggtcctcgc cgttaagccg cccagccgcg aacagcgctt cgccctcggc gatcaaggcg
     2161 acgaaccgcg cgtgggtctc cgccgtcatt ccgcgctcgg ccagacgccg ggccgcaaag
     2221 ccctccagca cccctcgcac ctcgatggcg tcgcggatct ggtcgctgga cacgcctcgg
     2281 gcggcataac cccgcgcgcc cagccgcacc acgagccctt cctgctccag actccgcaag
     2341 gccgtgcgca cgggcatgcg cgagacgccc agcgccgccg ctgtcgggat ttcggcgata
     2401 cgctcgccgc tcttgatctc gcccgaggcg atcatcttgc gaagagccat catcacgcgc
     2461 tggcccggct ttatgcgcgg catgtccatt cggggtctct ccctgctagc atcagggtta
     2521 ttgtatcatg agcggataca tatgtcaatg taccggagtt aacattggat ccaatcttga
     2581 cggatcgccg cgaagccgct aaattggatc caatactagt agaggaagct tccgcatggt
     2641 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2701 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     2761 gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     2821 gaccaccctg ggctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     2881 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2941 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3001 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3061 ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     3121 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
     3181 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3241 gagctaccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3301 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agattaatat
     3361 gactctagag acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     3421 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     3481 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
     3541 tcgtataatg tgtggatttt gagttaggat ccggcgagat tttcaggagc taaggaagct
     3601 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     3661 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     3721 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     3781 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     3841 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     3901 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     3961 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     4021 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     4081 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     4141 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc
     4201 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     4261 taaacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4321 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4381 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4441 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     4501 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4561 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4621 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4681 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4741 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4801 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4861 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4921 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4981 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5041 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     5101 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     5161 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg tggatccggc ttactaaaag
     5221 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     5281 tgtatacccg aagtatgtca aaaagaggtg tgctatgaag cagcgtatta cagtgacagt
     5341 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     5401 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     5461 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     5521 aacagggact ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     5581 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     5641 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     5701 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     5761 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     5821 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     5881 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     5941 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     6001 aagtggtgat atcgctagct agaggtaccg agctccaatt gggatccgga ctacaaggac
     6061 cacgacggcg actataagga ccacgacatc gactacaagg atgacgacga caagtgagga
     6121 tccggtgaag tgacccgcaa aaaaaaaggc caggggatcg ctcccccggc cttttttttg
     6181 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6241 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6301 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     6361 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     6421 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     6481 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     6541 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     6601 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagcaccc tttctcggtc
     6661 cttcaacgtt cctgacaacg agcctccttt tcgccaatcc atcgacaatc accgcgagtc
     6721 cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg gcccgcaaca
     6781 gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg ctgtcgccgg
     6841 cctgctcctc aagcacggcc ccaacagtga agtagctgat tgtcatcagc gcattgacgg
     6901 cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca
     6961 tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca
     7021 gcgcctgcct gaagctgcgg gcattcccga tcagaaatga gcgccagtcg tcgtcggctc
     7081 tcggcaccga atgcgtatga ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg
     7141 cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg aacccccaac cgttccgcca
     7201 gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa caggtccagg gcggcacgga
     7261 tcactgtatt cggctgcaac tttgtcatga ttgacacttt atcactgata aacataatat
     7321 gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt
     7381 ccggaggcca gacgtgaaac ccaacatacc cctgatcgta attctgagca ctgtcgcgct
     7441 cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt
     7501 tcactcgaac gacgtcaccg cccactatgg cattctgctg gcgctgtatg cgttggtgca
     7561 atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc ggccaatctt
     7621 gctcgtctcg ctggccggcg ccactgtcga ctacgccatc atggcgacag cgcctttcct
     7681 ttgggttctc tatatcgggc ggatcgtggc cggcatcacc ggggcgactg gggcggtagc
     7741 cggcgcttat attgccgata tcactgatgg cgatgagcgc gcgcggcact tcggcttcat
     7801 gagcgcctgt ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg
     7861 tttctccccc cacgctccgt tcttcgccgc ggcagccttg aacggcctca atttcctgac
     7921 gggctgtttc cttttgccgg agtcgcacaa aggcgaacgc cggccgttac gccgggaggc
     7981 tctcaacccg ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg ccgccctgat
     8041 ggcggtcttc ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt
     8101 cggcgaggat cgctttcact gggacgcgac cacgatcggc atttcgcttg ccgcatttgg
     8161 cattctgcat tcactcgccc aggcaatgat caccggccct gtagccgccc ggctcggcga
     8221 aaggcgggca ctcatgctcg gaatgattgc cgacggcaca ggctacatcc tgcttgcctt
     8281 cgcgacacgg ggatggatgg cgttcccgat catggtcctg cttgcttcgg gtggcatcgg
     8341 aatgccggcg ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc aggggcagct
     8401 gcaaggctca ctggcggcgc tcaccagcct gacctcgatc gtcggacccc tcctcttcac
     8461 ggcgatctat gcggcttcta taacaacgtg gaacgggtgg gcatggattg caggcgctgc
     8521 cctctacttg ctctgcctgc cggcgctgcg tcgcgggctt tggagcggcg cagggcaacg
     8581 agccgatcgc tgatcgtgga aacgataggc ctatgccatg cgggtcaagg cgacttccgg
     8641 caagctatac gcgccctaga attgtcaatt ttaatcctct gtttatcggc agttcgtaga
     8701 gcgcgccgtg cgtcccgagc gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg
     8761 ttcctgaaat gccagtaaag cgctggctgc tgaaccccca gccggaactg accccacaag
     8821 gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct
     8881 gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga
     8941 atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga
     9001 gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat
     9061 gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg
     9121 gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga
     9181 ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag
     9241 gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag
     9301 ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac
     9361 ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt
     9421 cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt
     9481 gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc
     9541 cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg
     9601 tttcagcaac gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt
     9661 ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc
     9721 tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc
     9781 agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga
     9841 gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc
     9901 ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa
     9961 cgtccgattc attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc
    10021 ttattcctga tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat
    10081 gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc
    10141 ctgcacgaat accagctccg cgaagtcgct cttcttgatg gagcgcatgg ggacgtgctt
    10201 ggcaatcacg cgcacccccc ggccgtttta gcggctaaaa aagtcatggc tctgccctcg
    10261 ggcggaccac gcccatcatg accttgccaa gctcgtcctg cttctcttcg atcttcgcca
    10321 gcagggcgag gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg gtgagccaga
    10381 gtttcagcag gccgcccagg cggcccaggt cgccattgat gcgggccagc tcgcggacgt
    10441 gctcatagtc cacgacgccc gtgattttgt agccctggcc gacggccagc aggtaggcct
    10501 acaggctcat gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc
    10561 agtacacctt gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct
    10621 tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga
    10681 gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta
    10741 cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc
    10801 tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa
    10861 tgaccccgaa gcagggttat gcagcggaaa agatccgtc
//
