LOCUS       sequence-99640-e       12420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses chimeric transactivator LexA_DBD:QF2_AD in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12420)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 12420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pCaSpeR-act5cB-LexAQF
FEATURES             Location/Qualifiers
     source          1..12420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..586)
                     /label=P element 5' end
     gene            complement(597..4725)
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     CDS             complement(join(1364..1978,2049..2180,2401..2716,2778..3432,
                     3507..3780,4175..4246))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4698..4716
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     promoter        4846..7303
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5c promoter"
     CDS             7322..7342
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7352..7603,7604..7957)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     misc_feature    join(9428..9629,9630..9660)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(10197..10216)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10316..10338
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10376..10394)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10462..10566
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10567..10635,10636..11427)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10785..10804)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11598..12186
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12087..12106
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttaattctag
      601 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
      661 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca tctacacaag
      721 gaacaaacac tggatgtcac tttcagttca aattgtaacg ctaatcactc cgaacaggtc
      781 acaaaaaatt accttaaaaa gtcataatat taaattagaa taaatatagc tgtgagggaa
      841 atatatacaa atatattgga gcaaataaat tgtacataca aatatttatt actaatttct
      901 attgagacga aatgaaccac tcggaaccat ttgagcgaac cgaatcgcgc ggaactaacg
      961 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     1021 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     1081 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     1141 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     1201 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     1261 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     1321 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     1381 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     1441 ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc
     1501 ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg
     1561 cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc
     1621 atttgaggta tactggcacc gagcccgagt tcaagaagaa gccgccaaag agcaggaatg
     1681 gtatgataac cggcggaccc acagacagcg ccatcgaggt cgaggagctg gcgcaggata
     1741 ttagatatcc gaaggacgtt gacacattgg ccaccagagt gaccagcgcc aggcagttga
     1801 agaagtgcag cactccggcc cgcagtccga tcatcggata ggcaatcgcc gtgaagacca
     1861 gtggcactgt gagaaaaagc ggtaattcgg caatcgtttt gcccagaaag tatgtgtcac
     1921 agcgataaag tcgacttcgg gcctccctca taaaaactgg cagctctgag gtgaacacct
     1981 aaatcgaatc gattcattag aaagttagta aattattaat atgcaaatgt attctaaaca
     2041 agacttacat ttatcgtggc aaagacgttt tgaaaggtca tgttggtcag gaagaggaag
     2101 atggctccgt tgatattcat cacgcccact tgcgtgagtt gttggcccaa aaagatgagg
     2161 ccaatcaaga tggcaaccat ctgcaaatta aaatgttact cgcatctcat taatattcat
     2221 atcttcaaca tgttcgcgag ttaaatgaaa tttatttatt ttctgcaaaa ctataaacta
     2281 tacatctcat tgaaaaaaac taagaagggt gtggaatcag gcaattctaa ctaaaatcta
     2341 gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac
     2401 cgttgtctga ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga
     2461 tcgccacagg accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc
     2521 cggctgctcc agtggcttct ccaaattttt ggtggccaac aactgctcca tatcccgggc
     2581 tactttgcta atagcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc
     2641 ccgtccgggc acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg
     2701 acactgggca cccacgctgg ataggagttg agatgtaatg taatgctaga tacccttaat
     2761 aaacacatcg aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag
     2821 ctaccctgcc ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaagacg
     2881 gctgatgaat ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga
     2941 cgacgctgtg ggcggtaaat gagtccagtc cggaggtggg ctcatcgcag atcagaagcg
     3001 gcggatcggt tagtgcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac
     3061 ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct
     3121 ggatcacctg atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca
     3181 ccatggcctg gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct
     3241 gctggacata ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca
     3301 gtcgcatccc ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca
     3361 gcagggtcgt ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg
     3421 ccacgccgca aactgagttt caaattggta attggaccct ttattaagat ttcacacaga
     3481 tcagccgact gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat
     3541 gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc
     3601 cggctgattg accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc
     3661 tagttggccg ctccctgaac cggagtcctc cggcggactg ggtggcagga gcgtgccgta
     3721 gtttttggcc tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc
     3781 ctgcaatgat aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac
     3841 aaaacgaagt atcctacgaa gtaggtttat acttttattt attttttgtg catctaggat
     3901 cagcttaaaa tatctggttg ttatattttt tgtaaaaaag aatgtagtcg aaaatgaatg
     3961 cctttagatg tcttgatcat gatatgatct taaaaattgt cttatatagc gagcacagct
     4021 accagaataa tctgtttcgt gtcactattt gtttgtgcga ttgcggtttg ggatttttgt
     4081 gggtcgcagt tctcacgccg cagacaattt gatgttgcaa tcgcagttcc tatagatcaa
     4141 gtgaacttaa gatgtatgca catgtactac tcacattgtt cagatgctcg gcagatgggt
     4201 gtttgctgcc tccgcgaatt aatagctcct gatcctcttg gcccattgcc gggatttttc
     4261 acactttccc ctgcttaccc acccaaaacc aatcaccacc ccaatcactc aaaaaacaaa
     4321 caaaaataag aagcgagagg agttttggca cagcactttg tgtttaattg atggcgtaaa
     4381 ccgcttggag cttcgtcacg aaaccgctga caaagtgcaa ctgaaggcgg acattgacgc
     4441 taggtaacgc tacaaacggt ggcgaaagag atagcggacg cagcggcgaa agagacggcg
     4501 atatttctgt ggacagagaa ggaggcaaac agcgctgact ttgagtggaa tgtcattttg
     4561 agtgagaggt aatcgaaaga acctggtaca tcaaataccc ttggatcgaa gtaaatttaa
     4621 aactgatcag ataagttcaa tgatatccag tgcagtaaaa aaaaaaaatg ttttttttat
     4681 ctactttccg caaaaatggg ttttattaac ttacatacat actagaattc tatattctaa
     4741 aaacacaaat gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt
     4801 tgcagatgca cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg
     4861 ttatgtggcc cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca
     4921 aacaccaaat acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct
     4981 tcgggcggag taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac
     5041 aatccaatgc caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg
     5101 cctggcgatc gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa
     5161 agactcgtcg gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg
     5221 ctgaggaatt tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta
     5281 tacgaaaatc agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg
     5341 caaaatatgt tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc
     5401 ttatgtattt ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaca
     5461 ggctgatttc tgcgtctttt gccgaacgca gtgcatgtgc ggttgttgtt ttttggaata
     5521 gtttcaattt tcggactgtc cgctttgatt tcagtttctt ggcttattca aaaagcaaag
     5581 taaagccaaa aaagcgagat ggcaatacca aatgcggcaa aacggtagtg gaaggaaagg
     5641 ggtgcggggc agcggaagga agggtggggc ggggcgtggc ggggtctgtg gctgggcgcg
     5701 acgtcaccga cgttggagcc actcctttga ccatgtgtgc gtgtgtgtat tattcgtgtc
     5761 tcgccactcg ccggttgttt ttttcttttt atctcgctct ctctagcgcc atctcgtacg
     5821 catgctcaac gcaccgcatg ttgccgtgtc ctttatgcgt cattttggct cgaaataggc
     5881 aattatttaa acaaagatta gtcaacgaaa acgctaaaat aaataagtct acaatatggt
     5941 tacttattgc catgtgtgtg cagccaacga tagcaacaaa agcaacaaca cagtggcttt
     6001 ccctctttca ctttttgttt gcaagcgcgt gcgagcaaga cggcacgacc ggcaaacgca
     6061 attacgctga caaagagcag acgaagtttt ggccgaaaaa catcaaggcg cctgatacga
     6121 atgcatttgc aataacaatt gcgatattta atattgttta tgaagctgtt tgacttcaaa
     6181 acacacaaaa aaaaaaataa aacaaattat ttgaaagaga attaggaatc ggacagctta
     6241 tcgttacggg ctaacagcac accgagacga aatagcttac ctgacgtcac agcctctgga
     6301 agaactgccg ccaagcagac gatgcagagg acgacacata gagtagcgga gtaggccagc
     6361 gtagtacgca tgtgcttgtg tgtgaggcgt ctctctcttc gtctcctgtt tgcgcaaacg
     6421 catagactgc actgagaaaa tcgattacct attttttatg aatgaatatt tgcactatta
     6481 ctattcaaaa ctattaagat agcaatcaca ttcaatagcc aaatactata ccacctgagc
     6541 gatgcaacga aatgatcaat ttgagcaaaa atgctgcata tttaggacgg catcattata
     6601 gaaatgcttc ttgctgtgta cttttctctc gtctggcagc tgtttcgccg ttattgttaa
     6661 aaccggctta agttaggtgt gttttctacg actagtgaat gccctactag aagatgtgtg
     6721 ttgcacaaaa tgtccctgga ataaccaatt tgaagtgcag atagcagtaa acgtaagcta
     6781 atatgaatat tatttaactg taatgtttta atatcgctgg acattactaa taaacccact
     6841 ataaacacat gtacatatgt atgttttggc atacaatgag tagttgggga aaaaatgtgt
     6901 aaaagcaccg tgaccatcac agcataaaga taaccagctg aagtatcgaa tatgagtaac
     6961 ccccaaattg aatcacatgc cgcaactgat aggacccatg gaagtacact cttcatggcg
     7021 atatacaaga cacacacaag cacgaacacc cagttgcgga ggaaattctc cgtaaatgaa
     7081 aacccaatcg gcgaacaatt catacccata tatggtaaaa gttttgaacg cgacttgaga
     7141 gcggagagca ttgcggctga taaggtttta gcgctaagcg ggctttataa aacgggctgc
     7201 gggaccagtt ttcatatcac taccgtttga gttcttgtgc tgtgtggata ctcctcccga
     7261 cacaaagccg ctccatcagc cagcagtcgt ctaatccaga gaccccggat ccaaaatgcc
     7321 acccaagaag aagcgaaaag tagaagatcc aatgaaggct ctcacggccc gacaacagga
     7381 agtttttgat ttgatacggg atcatatatc ccaaacgggt atgcctccga cccgcgcaga
     7441 gatagcacag cgactgggct ttcgatcgcc taacgccgcg gaggagcact tgaaggcact
     7501 ggcccgcaag ggtgtcattg aaatcgtgtc cggtgcgagc cgcggaatcc ggctgttgca
     7561 ggaggaggaa gagggcctgc cactggtggg acgcgtggcc gctggcgagc cgctgctggc
     7621 ccagcaacac atagagggac actatcaggt ggacccctcc ttgtttaagc caaatgctga
     7681 tttcctgttg cgggtgtcgg gaatgtccat gaaggacatc ggtattatgg atggtgacct
     7741 cctcgccgtc cataagacac aggatgtcag gaacggccag gtagtcgttg ccaggataga
     7801 cgatgaggtc actgtgaaac gtctcaagaa gcaaggcaat aaggtcgagc tgctgccgga
     7861 gaatagcgag ttcaagccga tcgtggtgga tctgcgacag cagtccttta ctatcgaggg
     7921 cttggccgtg ggtgtgatcc gcaacggaga ttggctggga cgtcagttgg agctccctcc
     7981 taccgccacg gctacggcct cgataatgcc gcacgtgatg gagcagcctc tcagtaccag
     8041 cattaacccc gtcaacgacc gcttcaacgg tattcccaac cccactccgt ataactccga
     8101 tgcagctctc gatgctatca ctcagaccaa cgattatgga agcgtaaata cacatggtat
     8161 cctctctact tacccgccac cggctacgca ccttaatgaa gcttccgtcg ctctcgctcc
     8221 cggtggcgcc cccccccgac cgcctcctcc gtatgttgac agcacgacca atcacccgcc
     8281 gtaccactcg aatctggttc caatggcgaa ctttggttac tcgaccgttg attacgatgc
     8341 catggttgac gatttggcta gcattgaata cacggacgct gtggatgtcg acccacagtt
     8401 tatgaccaat ctgggattcg ttcctggatg taacttctcc gacattaata catacgaaca
     8461 gtgagcggcc gcgatccgtc gaccatgaag atcaagatca ttgccccgcc agagcgcaag
     8521 tactctgtct ggatcggtgg ctccatcctg gcttcgctgt ccaccttcca gcagatgtgg
     8581 atctccaagc aggagtacga cgagtccggc ccctccattg tgcaccgcaa gtgcttctaa
     8641 gaaggatcgc ttgtctgggc aagaggatca ggatcgggat ggtcttgatt ctgctggagg
     8701 aggaggagga gaagtcgagg aagcagcagc gaaagtgcaa gtgcgagtgg tggaagtttg
     8761 gagtgcagca caacaaaatc aacaacaaca ccaactacaa gatgaaaaga gcggaaccac
     8821 ctgcacacca tcatcactat catcatcgtt ttgggcgcat gttgtgtggt tccagcgtat
     8881 taatataatt aatttattcc acatgagata tgatatgata tactatgtat tttttgtttt
     8941 ttttttattt gtaaaccttt aatataacaa gaactacaaa aaatgaaaat gagcgaaaat
     9001 gcatattctg ccattccaca cacacaccaa caacacccaa cacacgcaca cccacaagct
     9061 tacacacaca cattcgcggc atgacaagga catcaagata aagaagaact taaagaagat
     9121 atttcccaaa gcgcaaaaag aacacacaca cattgcaaaa cacaaacaac acactagcgt
     9181 tttgtacaat tcgtcagcaa ccttatgtat tattttttaa ttatgatgta attataaaca
     9241 aagtgaaaca aaaatatgaa aacaaaaagg aaaatcaaat ctgtcttctc tttctcccgc
     9301 tctcctcgct ctctgctgct aacctcgccc tctcctctct catctttttg tctgtctctc
     9361 ttcacatttt tgccggccgg caaaataata acccacacac actcacactt ggctgcagcc
     9421 caagctttgc gtactcgcaa attattaaaa ataaaacttt aaaaataatt tcgtctaatt
     9481 aatattatga gttaattcaa accccacgga catgctaagg gttaatcaac aatcatatcg
     9541 ctgtctcact cagactcaat acgacactca gaatactatt cctttcactc gcacttattg
     9601 caagcatacg ttaagtggat gtctcttgcc gacgggacca ccttatgtta tttcatcatg
     9661 gtctggccat tctcatcgtg agcttccggg tgctcgcata tctggctcta agacttcggg
     9721 cccgacgcaa ggagtagccg acatatatcc gaaataactg cttgtttttt tttttaccat
     9781 tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg
     9841 ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca
     9901 agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa
     9961 aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa
    10021 tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct
    10081 gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt
    10141 cgttagttcc gcgcgattcg gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
    10201 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
    10261 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
    10321 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
    10381 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
    10441 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
    10501 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
    10561 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
    10621 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
    10681 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
    10741 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
    10801 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    10861 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    10921 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    10981 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    11041 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    11101 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    11161 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    11221 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    11281 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    11341 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    11401 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    11461 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    11521 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    11581 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    11641 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    11701 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
    11761 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    11821 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    11881 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    11941 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
    12001 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    12061 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    12121 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    12181 tggaaaaacg ccttcttctt gaactcgggc tcggtgccag tatacctcaa atggttgtcg
    12241 tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg
    12301 gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc gggcaaggtc
    12361 atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt gggtctggcc
//
