LOCUS       sequence-99642-e       12133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer trap version of QF2, analogous to pGAWB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12133)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 12133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pQF2WB
FEATURES             Location/Qualifiers
     source          1..12133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1507..1523)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1507..1523)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     gap             5949..6158
                     /estimated_length=210
     primer_bind     complement(6219..6238)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6220..6238)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6248..6265)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6248..6264)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6257..6279)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(6405..6860)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6542..6563)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6754..6773
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6887..6991
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6992..7060,7061..7852)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8023..8611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8765..8782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8899..8920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8935..8940,8941..8958,8959..8965)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8973..8989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8978..9000
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8997..9013
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8997..9013
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9032..9052
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9034..9052
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(9141..9342,9343..9373)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(9910..9929)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10029..10051
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10089..10107)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10175..10279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10280..10348,10349..11140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11311..11899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg cggccgccaa catgccaccc aagcgcaaaa cgcttaacgc
      181 tgcggctgag gctaacgctc atgccgacgg acacgccgac ggaaacgccg acggacacgt
      241 ggccaatacg gccgcgtcct cgaataatgc gaggttcgct gatctcacta acatcgatac
      301 tccgggtctg ggacccacaa ctacgaccct gctcgtggaa ccagcacgct caaagcgtca
      361 acgagtgtcc cgcgcatgcg accagtgccg tgcagcccga gagaaatgcg acggaataca
      421 gcctgcgtgt ttcccgtgcg tttcccaggg aaggtcctgc acttatcagg cttcgccgaa
      481 aaagagggga gttcaaaccg gttatattcg tacgctggag ctcgccctcg cctggatgtt
      541 tgaaaatgtc gcgcgttccg aagatgcctt gcataacctc ctcgtccgtg acgccggaca
      601 aggatcagct ctgctcgttg gtaaagattc gccggctgcc gagcgactcc atgcccgttg
      661 ggctactagc cgtgtcaata agagcattac ccgcctcctc cgtcagttgg agctccctcc
      721 taccgccacg gctacggcct cgataatgcc gcacgtgatg gagcagcctc tcagtaccag
      781 cattaacccc gtcaacgacc gcttcaacgg tattcccaac cccactccgt ataactccga
      841 tgcagctctc gatgctatca ctcagaccaa cgattatgga agcgtaaata cacatggtat
      901 cctctctact tacccgccac cggctacgca ccttaatgaa gcttccgtcg ctctcgctcc
      961 cggtggcgcc cccccccgac cgcctcctcc gtatgttgac agcacgacca atcacccgcc
     1021 gtaccactcg aatctggttc caatggcgaa ctttggttac tcgaccgttg attacgatgc
     1081 catggttgac gatttggcta gcattgaata cacggacgct gtggatgtcg acccacagtt
     1141 tatgaccaat ctgggattcg ttcctggatg taacttctcc gacattaata catacgaaca
     1201 gtgatgacgt ctatcgatac cgtcgactaa agccaaatag aaaattattc agttcctggc
     1261 ttaagttttt aaaagtgata ttatttattt ggttgtaacc aaccaaaaga atgtaaataa
     1321 ctaatacata attatgttag ttttaagtta gcaacaaatt gattttagct atattagcta
     1381 cttggttaat aaatagaata tatttattta aagataattg cgtttttatt gtcagggagt
     1441 gagtttgctt aaaaactcgt ttagatccgc ggccgcagct tgatatcgaa ttcctgcagc
     1501 ccgggggatc cactagttct agagcggccc cccgttattc tctattcgtt ttgtgactct
     1561 ccctctctgt actattgctc tctcactctg tcgcacagta aacggcacac tattctcgtt
     1621 gcttcgagag agcgcgcctc gaatgttcgc gaaaagagcg ccggagtata aatagaggcg
     1681 cttcgtcgac ggagcgtcaa ttcaattcaa acaagcaaag tgaacacatc gcgaagcgta
     1741 agctgagcaa acaaacaagc gcagctgaac aagctaaaca atctgcaata aagtgcaagt
     1801 taaagtgaat caattaaaag taaccaacaa ccaagtaatt aaactaaaaa ctgcaactac
     1861 tgaaatcaac caagaagtca ttattgaaga caagaagaga actctgaata ggtcgatagc
     1921 gtcaatgtcc gccttcagtt gcactttgtc agcggtttcg tgacgaagct ccaagcggtt
     1981 tacgccatca attaaacaca aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt
     2041 tgttttttga gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga
     2101 aaaatcccgg caatgggcca agaggatcag gagctattaa ttcgcggagg cagcaaacac
     2161 ccatctgccg agcatctgaa caatgtgagt agtacatgtg catacatctt aagttcactt
     2221 gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac
     2281 aaaaatccca aaccgcaatc gcacaaacaa atagtgacac gaaacagatt attctggtag
     2341 ctgtgctcgc tatataagac aatttttaag atcatatcat gatcaagaca tctaaaggca
     2401 ttcattttcg actacattct tttttacaaa aaatataaca accagatatt ttaagcttcg
     2461 actctagcta gatgcacaaa aaataaataa aagtataaac ctacttcgta ggatacttcg
     2521 ttttgttcgg ggttagatga gcataacgct tgtagttgat atttgagatc ccctatcatt
     2581 gcagggtgac agcggacgct tcgcagagct gcattaacca gggcttcggg caggccaaaa
     2641 actacggcac gctcctgcca cccagtccgc cggaggactc cggttcaggg agcggccaac
     2701 tagccgagaa cctcacctat gcctggcaca atatggacat ctttggggcg gtcaatcagc
     2761 cgggctccgg atggcggcag ctggtcaacc ggacacgcgg actattctgc aacgagcgac
     2821 acataccggc gcccaggaaa catttgctca agaacggtga gtttctattc gcagtcggct
     2881 gatctgtgtg aaatcttaat aaagggtcca attaccaatt tgaaactcag tttgcggcgt
     2941 ggcctatccg ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa agacgaccct
     3001 gctgaatgcc cttgcctttc gatcgccgca gggcatccaa gtatcgccat ccgggatgcg
     3061 actgctcaat ggccaacctg tggacgccaa ggagatgcag gccaggtgcg cctatgtcca
     3121 gcaggatgac ctctttatcg gctccctaac ggccagggaa cacctgattt tccaggccat
     3181 ggtgcggatg ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat
     3241 ccaggagctt tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa
     3301 aggtctgtcc ggcggagaaa ggaagcgtct ggcattcgcc tccgaggcac taaccgatcc
     3361 gccgcttctg atctgcgatg agcccacctc cggactggac tcatttaccg cccacagcgt
     3421 cgtccaggtg ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga ccattcatca
     3481 gccgtcttcc gagctgtttg agctctttga caagatcctt ctgatggccg agggcagggt
     3541 agctttcttg ggcactccca gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt
     3601 ttattaaggg tatctagcat tacattacat ctcaactcct atccagcgtg ggtgcccagt
     3661 gtcctaccaa ctacaatccg gcggactttt acgtacaggt gttggccgtt gtgcccggac
     3721 gggagatcga gtcccgtgat cggatcgcca agatatgcga caattttgct attagcaaag
     3781 tagcccggga tatggagcag ttgttggcca ccaaaaattt ggagaagcca ctggagcagc
     3841 cggagaatgg gtacacctac aaggccacct ggttcatgca gttccgggcg gtcctgtggc
     3901 gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt attcagacaa
     3961 cggtgagtgg ttccagtgga aacaaatgat ataacgctta caattcttgg aaacaaattc
     4021 gctagatttt agttagaatt gcctgattcc acacccttct tagttttttt caatgagatg
     4081 tatagtttat agttttgcag aaaataaata aatttcattt aactcgcgaa catgttgaag
     4141 atatgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc catcttgatt
     4201 ggcctcatct ttttgggcca acaactcacg caagtgggcg tgatgaatat caacggagcc
     4261 atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat aaatgtaagt
     4321 cttgtttaga atacatttgc atattaataa tttactaact ttctaatgaa tcgattcgat
     4381 ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc gactttatcg
     4441 ctgtgacaca tactttctgg gcaaaacgat tgccgaatta ccgctttttc tcacagtgcc
     4501 actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag tgctgcactt
     4561 cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct tcggatatct
     4621 aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc cggttatcat
     4681 accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag tatacctcaa
     4741 atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg
     4801 ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc
     4861 gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt
     4921 gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg
     4981 ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt tttttttacc
     5041 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5101 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5161 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5221 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5281 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5341 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5401 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     5461 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     5521 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     5581 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     5641 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     5701 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     5761 aactagttct ctctctctct ctcttatcta tcgctacttg gttggcgcgc tctcgcgctc
     5821 tctttgtgtg cgtgtgggca gtgtgttttt tgttgttttg cgctttatgt gttgtatttt
     5881 gtgtgtttgg ccgaagtatt taaaacaaaa gtgcagcgga aatagttaat aacaaaatat
     5941 tagtcgacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga attagcttgg ctgcaggtcg
     6181 acctcgaggg gccgccaccg cggtggagct ccaattcgcc ctatagtgag tcgtattacg
     6241 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     6301 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     6361 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggaaattg taagcgttaa
     6421 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     6481 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     6541 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     6601 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     6661 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     6721 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     6781 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     6841 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6901 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6961 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7021 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7081 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     7141 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     7201 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     7261 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7321 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7381 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7441 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7501 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     7561 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     7621 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     7681 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     7741 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     7801 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     7861 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     7921 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7981 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8041 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8101 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8161 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8221 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8281 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8341 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8401 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8461 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8521 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8581 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8641 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     8701 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     8761 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     8821 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     8881 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     8941 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9001 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     9061 gctgggtacc cgcccgggat cagatccgcg gccggccgcg cataggccac tagtggatct
     9121 ggatcctcta gctagagctt tgcgtactcg caaattatta aaaataaaac tttaaaaata
     9181 atttcgtcta attaatatta tgagttaatt caaaccccac ggacatgcta agggttaatc
     9241 aacaatcata tcgctgtctc actcagactc aatacgacac tcagaatact attcctttca
     9301 ctcgcactta ttgcaagcat acgttaagtg gatgtctctt gccgacggga ccaccttatg
     9361 ttatttcatc atggtctggc cattctcatc gtgagcttcc gggtgctcgc atatctggct
     9421 ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa ctgcttgttt
     9481 ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt
     9541 aattatattt gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc
     9601 gaacatcccc acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca
     9661 taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata
     9721 catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag
     9781 ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc
     9841 ttggagcgac tgtcgttagt tccgcgcgat tcggtgcggt atttcacacc gcatatggtg
     9901 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     9961 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
    10021 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
    10081 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
    10141 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    10201 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    10261 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    10321 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    10381 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
    10441 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    10501 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
    10561 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    10621 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    10681 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    10741 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    10801 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    10861 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    10921 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    10981 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    11041 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    11101 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    11161 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    11221 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    11281 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    11341 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    11401 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
    11461 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    11521 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    11581 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    11641 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    11701 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    11761 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    11821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    11881 ggggcggagc ctatggaaaa acgccttctt cttgaactcg ggctcggtgc cagtatacct
    11941 caaatggttg tcgtacctct catggttccg ttacgccaac gagggtctgc tgattaacca
    12001 atgggcggac gtggagccgg gcgaaattag ctgcacatcg tcgaacacca cgtgccccag
    12061 ttcgggcaag gtcatcctgg agacgcttaa cttctccgcc gccgatctgc cgctggacta
    12121 cgtgggtctg gcc
//
