LOCUS       sequence-99643-e       12139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer trap version of QF2w, analogous to pGAWB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12139)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 12139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pQF2wWB
FEATURES             Location/Qualifiers
     source          1..12139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1513..1529)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1513..1529)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     gap             5955..6164
                     /estimated_length=210
     primer_bind     complement(6225..6244)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6226..6244)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6254..6271)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6254..6270)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6263..6285)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(6411..6866)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6548..6569)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6760..6779
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6893..6997
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6998..7066,7067..7858)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8029..8617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8771..8788
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8905..8926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8941..8946,8947..8964,8965..8971)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8979..8995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8984..9006
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9003..9019
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9003..9019
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9038..9058
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9040..9058
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(9147..9348,9349..9379)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(9916..9935)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10035..10057
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10095..10113)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10181..10285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10286..10354,10355..11146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11317..11905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg cggccgccaa catgccaccc aagcgcaaaa cgcttaacgc
      181 tgcggctgag gctaacgctc atgccgacgg acacgccgac ggaaacgccg acggacacgt
      241 ggccaatacg gccgcgtcct cgaataatgc gaggttcgct gatctcacta acatcgatac
      301 tccgggtctg ggacccacaa ctacgaccct gctcgtggaa ccagcacgct caaagcgtca
      361 acgagtgtcc cgcgcatgcg accagtgccg tgcagcccga gagaaatgcg acggaataca
      421 gcctgcgtgt ttcccgtgcg tttcccaggg aaggtcctgc acttatcagg cttcgccgaa
      481 aaagagggga gttcaaaccg gttatattcg tacgctggag ctcgccctcg cctggatgtt
      541 tgaaaatgtc gcgcgttccg aagatgcctt gcataacctc ctcgtccgtg acgccggaca
      601 aggatcagct ctgctcgttg gtaaagattc gccggctgcc gagcgactcc atgcccgttg
      661 ggctactagc cgtgtcaata agagcattac ccgcctcctc cgtcagttgg agctccctcc
      721 taccgccacg gctacggcct cgataatgcc gcacgtgatg gagcagcctc tcagtaccag
      781 cattaacccc gtcaacgacc gcttcaacgg tattcccaac cccactccgt ataactccga
      841 tgcagctctc gatgctatca ctcagaccaa cgattatgga agcgtaaata cacatggtat
      901 cctctctact tacccgccac cggctacgca ccttaatgaa gcttccgtcg ctctcgctcc
      961 cggtggcgcc cccccccgac cgcctcctcc gtatgttgac agcacgacca atcacccgcc
     1021 gtaccactcg aatctggttc caatggcgaa ctttggttac tcgaccgttg attacgatgc
     1081 catggttgac gatttggcta gcattgaata cacggacgct gtggatgtcg acccacagtt
     1141 tatgaccaat ctgggattcg ttcctggatg taacttctcc gacattaata catacaaaaa
     1201 gaagaaatga tgacgtctat cgataccgtc gactaaagcc aaatagaaaa ttattcagtt
     1261 cctggcttaa gtttttaaaa gtgatattat ttatttggtt gtaaccaacc aaaagaatgt
     1321 aaataactaa tacataatta tgttagtttt aagttagcaa caaattgatt ttagctatat
     1381 tagctacttg gttaataaat agaatatatt tatttaaaga taattgcgtt tttattgtca
     1441 gggagtgagt ttgcttaaaa actcgtttag atccgcggcc gcagcttgat atcgaattcc
     1501 tgcagcccgg gggatccact agttctagag cggccccccg ttattctcta ttcgttttgt
     1561 gactctccct ctctgtacta ttgctctctc actctgtcgc acagtaaacg gcacactatt
     1621 ctcgttgctt cgagagagcg cgcctcgaat gttcgcgaaa agagcgccgg agtataaata
     1681 gaggcgcttc gtcgacggag cgtcaattca attcaaacaa gcaaagtgaa cacatcgcga
     1741 agcgtaagct gagcaaacaa acaagcgcag ctgaacaagc taaacaatct gcaataaagt
     1801 gcaagttaaa gtgaatcaat taaaagtaac caacaaccaa gtaattaaac taaaaactgc
     1861 aactactgaa atcaaccaag aagtcattat tgaagacaag aagagaactc tgaataggtc
     1921 gatagcgtca atgtccgcct tcagttgcac tttgtcagcg gtttcgtgac gaagctccaa
     1981 gcggtttacg ccatcaatta aacacaaagt gctgtgccaa aactcctctc gcttcttatt
     2041 tttgtttgtt ttttgagtga ttggggtggt gattggtttt gggtgggtaa gcaggggaaa
     2101 gtgtgaaaaa tcccggcaat gggccaagag gatcaggagc tattaattcg cggaggcagc
     2161 aaacacccat ctgccgagca tctgaacaat gtgagtagta catgtgcata catcttaagt
     2221 tcacttgatc tataggaact gcgattgcaa catcaaattg tctgcggcgt gagaactgcg
     2281 acccacaaaa atcccaaacc gcaatcgcac aaacaaatag tgacacgaaa cagattattc
     2341 tggtagctgt gctcgctata taagacaatt tttaagatca tatcatgatc aagacatcta
     2401 aaggcattca ttttcgacta cattcttttt tacaaaaaat ataacaacca gatattttaa
     2461 gcttcgactc tagctagatg cacaaaaaat aaataaaagt ataaacctac ttcgtaggat
     2521 acttcgtttt gttcggggtt agatgagcat aacgcttgta gttgatattt gagatcccct
     2581 atcattgcag ggtgacagcg gacgcttcgc agagctgcat taaccagggc ttcgggcagg
     2641 ccaaaaacta cggcacgctc ctgccaccca gtccgccgga ggactccggt tcagggagcg
     2701 gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt ggggcggtca
     2761 atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta ttctgcaacg
     2821 agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt ctattcgcag
     2881 tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa actcagtttg
     2941 cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg ccggaaagac
     3001 gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat cgccatccgg
     3061 gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca ggtgcgccta
     3121 tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc tgattttcca
     3181 ggccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc gcgtggatca
     3241 ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg tgcccggcag
     3301 ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg aggcactaac
     3361 cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcat ttaccgccca
     3421 cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca tcctgaccat
     3481 tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga tggccgaggg
     3541 cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct agtgagttcg
     3601 atgtgtttat taagggtatc tagcattaca ttacatctca actcctatcc agcgtgggtg
     3661 cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg gccgttgtgc
     3721 ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat tttgctatta
     3781 gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatttggag aagccactgg
     3841 agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc cgggcggtcc
     3901 tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg cgacttattc
     3961 agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat tcttggaaac
     4021 aaattcgcta gattttagtt agaattgcct gattccacac ccttcttagt ttttttcaat
     4081 gagatgtata gtttatagtt ttgcagaaaa taaataaatt tcatttaact cgcgaacatg
     4141 ttgaagatat gaatattaat gagatgcgag taacatttta atttgcagat ggttgccatc
     4201 ttgattggcc tcatcttttt gggccaacaa ctcacgcaag tgggcgtgat gaatatcaac
     4261 ggagccatct tcctcttcct gaccaacatg acctttcaaa acgtctttgc cacgataaat
     4321 gtaagtcttg tttagaatac atttgcatat taataattta ctaactttct aatgaatcga
     4381 ttcgatttag gtgttcacct cagagctgcc agtttttatg agggaggccc gaagtcgact
     4441 ttatcgctgt gacacatact ttctgggcaa aacgattgcc gaattaccgc tttttctcac
     4501 agtgccactg gtcttcacgg cgattgccta tccgatgatc ggactgcggg ccggagtgct
     4561 gcacttcttc aactgcctgg cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg
     4621 atatctaata tcctgcgcca gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt
     4681 tatcatacca ttcctgctct ttggcggctt cttcttgaac tcgggctcgg tgccagtata
     4741 cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc tgctgattaa
     4801 ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc
     4861 cagttcgggc aaggtcatcc tggagacgct taacttctcc gccgccgatc tgccgctgga
     4921 ctacgtgggt ctggccattc tcatcgtgag cttccgggtg ctcgcatatc tggctctaag
     4981 acttcgggcc cgacgcaagg agtagccgac atatatccga aataactgct tgtttttttt
     5041 tttaccatta ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta
     5101 tatttgtgcc aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca
     5161 tccccacaag tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct
     5221 tacatcaaaa actcgtttat cgctacataa aacaccggga tatatttttt atatacatac
     5281 ttttcaaatc gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct
     5341 ctttcgctgt ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga
     5401 gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc
     5461 gtctcaatag aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt
     5521 gtatatattt ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa
     5581 ttttttgtga cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt
     5641 gtttgttcct tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat
     5701 atatcaaaaa taacaactat aataataaga atacatttaa tttagaaaat gcttggattt
     5761 cactggaact agttctctct ctctctctct tatctatcgc tacttggttg gcgcgctctc
     5821 gcgctctctt tgtgtgcgtg tgggcagtgt gttttttgtt gttttgcgct ttatgtgttg
     5881 tattttgtgt gtttggccga agtatttaaa acaaaagtgc agcggaaata gttaataaca
     5941 aaatattagt cgacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaatta gcttggctgc
     6181 aggtcgacct cgaggggccg ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt
     6241 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     6301 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     6361 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag
     6421 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     6481 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     6541 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     6601 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     6661 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     6721 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     6781 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     6841 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     6901 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6961 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7021 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7081 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7141 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7201 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7261 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7321 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7381 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7441 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7501 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7561 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7621 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7681 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7741 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7801 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7861 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7921 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7981 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8041 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8101 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8161 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8221 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8281 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8341 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8401 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8461 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8521 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8581 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8641 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8701 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8761 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     8821 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     8881 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     8941 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9001 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     9061 acaaaagctg ggtacccgcc cgggatcaga tccgcggccg gccgcgcata ggccactagt
     9121 ggatctggat cctctagcta gagctttgcg tactcgcaaa ttattaaaaa taaaacttta
     9181 aaaataattt cgtctaatta atattatgag ttaattcaaa ccccacggac atgctaaggg
     9241 ttaatcaaca atcatatcgc tgtctcactc agactcaata cgacactcag aatactattc
     9301 ctttcactcg cacttattgc aagcatacgt taagtggatg tctcttgccg acgggaccac
     9361 cttatgttat ttcatcatgg tctggccatt ctcatcgtga gcttccgggt gctcgcatat
     9421 ctggctctaa gacttcgggc ccgacgcaag gagtagccga catatatccg aaataactgc
     9481 ttgttttttt ttttaccatt attaccatcg tgtttactgt ttattgcccc ctcaaaaagc
     9541 taatgtaatt atatttgtgc caataaaaac aagatatgac ctatagaata caagtatttc
     9601 cccttcgaac atccccacaa gtagactttg gatttgtctt ctaaccaaaa gacttacaca
     9661 cctgcatacc ttacatcaaa aactcgttta tcgctacata aaacaccggg atatattttt
     9721 tatatacata cttttcaaat cgcgcgccct cttcataatt cacctccacc acaccacgtt
     9781 tcgtagttgc tctttcgctg tctcccaccc gctctccgca acacattcac cttttgttcg
     9841 acgaccttgg agcgactgtc gttagttccg cgcgattcgg tgcggtattt cacaccgcat
     9901 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     9961 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
    10021 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
    10081 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
    10141 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    10201 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    10261 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
    10321 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
    10381 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
    10441 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
    10501 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
    10561 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
    10621 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    10681 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    10741 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    10801 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    10861 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    10921 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    10981 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    11041 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    11101 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    11161 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    11221 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    11281 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    11341 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    11401 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    11461 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    11521 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    11581 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    11641 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    11701 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    11761 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    11821 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    11881 gtcagggggg cggagcctat ggaaaaacgc cttcttcttg aactcgggct cggtgccagt
    11941 atacctcaaa tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat
    12001 taaccaatgg gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg
    12061 ccccagttcg ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct
    12121 ggactacgtg ggtctggcc
//
