LOCUS       sequence-99649-e        1194 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-11a
FEATURES             Location/Qualifiers
     source          1..1194
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(378..380,381..1094)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDCPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(420..441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(681..700)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1028..1049
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1147..1164
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgggcag cagccatcat catcatcatc
       61 acggcagcgg cagcggctct gaccaggagg caaaaccttc aactgaggac ttgggggata
      121 agaaggaagg tgaatatatt aaactcaaag tcattggaca ggatagcagt gagattcact
      181 tcaaagtgaa aatgacaaca catctcaaga aactcaaaga atcatactgt caaagacagg
      241 gtgttccaat gaattcactc aggtttctct ttgagggtca gagaattgct gataatcata
      301 ctccaaaaga actgggaatg gaggaagaag atgtgattga agtttatcag gaacaaaccg
      361 gtggtgccat ggccaaagtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      421 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      481 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      541 tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc
      601 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      661 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      721 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      781 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      841 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      901 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgactgc cccgtgctgc
      961 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     1021 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1081 agctgtacaa gtaaggtacc ggatccgaat tcgagctccg tcgacaagct tgcggccgca
     1141 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaag
//
