LOCUS       sequence-99651-e        1277 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-13a
FEATURES             Location/Qualifiers
     source          1..1277
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..58
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             431..448
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(470..472,473..1186)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(512..533)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(773..792)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1120..1141
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1239..1256
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actttaagaa ggagatatac atatgggcag cagccatcat catcatcatc atcatcacaa
       61 cactagtagc aattccatgt tttgtacatt ttttgaaaaa catcaccgga agtgggacat
      121 actgttagaa aaaagcacgg gtgtgatgga agctatgaaa gtgacgagtg aggaaaagga
      181 acagctgagc acagcaatcg accgaatgaa tgaaggactg gacgcgttta tccagctgta
      241 taatgaatcg gaaattgatg aaccgcttat tcagcttgat gatgatacag ccgagttaat
      301 gaagcaggcc cgagatatgt acggccagga aaagctaaat gagaaattaa atacaattat
      361 taaacagatt ttatccatct cagtatctga agaaggcgaa aaagaaggtt ctggttctgg
      421 ttctggttct ctggtgcctc gtggcagcgg tggaggtgcc atggccaaag tgagcaaggg
      481 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      541 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      601 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
      661 cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
      721 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      781 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      841 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      901 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
      961 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1021 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1081 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1141 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaggta ccggatccga
     1201 attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag
     1261 atccggctgc taacaaa
//
