LOCUS       sequence-99656-e        1271 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-18a
FEATURES             Location/Qualifiers
     source          1..1271
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    140..164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(288..290,291..1004)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(330..351)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(591..610)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     938..959
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1057..1074
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtaccagcta ttgtaacata atcggtacgg gggtgaaaaa gctaacggaa aagggagcgg
       61 aaaagaatga tgtaagcgtg aaaaattttt tatcttatca cttgaaattg gaagggagat
      121 tctttattat aagaattgtg gaattgtgag cggataacaa ttcccaatta aaggaggaag
      181 gatcaatgat tcaaaaacga aagcggacag tttcgttcag acttgtgctt atgtgcacgc
      241 tgttatttgt cagtttgccg attacaaaaa catcagccat ggccaaagtg agcaagggcg
      301 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      361 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      421 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
      481 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
      541 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      601 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      661 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      721 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
      781 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      841 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
      901 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      961 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaggtacc ggatccgaat
     1021 tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgaaaa
     1081 gtttatacct tacctggaac aaatggttga aacatacgag gctaatatcg gcttattagg
     1141 aatagtccct gtactaataa aatcaggtgg atcagttgat cagtatattt tggacgaagc
     1201 tcggaaagaa tttggagatg acttgcttaa ttccacaatt aaattaaggg aaagaataaa
     1261 gcgatttgat g
//
