LOCUS       sequence-99663-e        1213 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-11b
FEATURES             Location/Qualifiers
     source          1..1213
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             30..52
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             71..88
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(407..409,410..1123)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(449..470)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(710..729)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1057..1078
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1176..1193
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttggacggaa cattcccctc tagaataatt ttgtttaact ttaagaagga gatataccat
       61 gggcagcagc catcatcatc atcatcacgg cagcggcagc ggctctgacc aggaggcaaa
      121 accttcaact gaggacttgg gggataagaa ggaaggtgaa tatattaaac tcaaagtcat
      181 tggacaggat agcagtgaga ttcacttcaa agtgaaaatg acaacacatc tcaagaaact
      241 caaagaatca tactgtcaaa gacagggtgt tccaatgaat tcactcaggt ttctctttga
      301 gggtcagaga attgctgata atcatactcc aaaagaactg ggaatggagg aagaagatgt
      361 gattgaagtt tatcaggaac aaaccggtgg tcatatggct agcaaagtga gcaagggcga
      421 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      481 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      541 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
      601 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
      661 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      721 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      781 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      841 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
      901 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
      961 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1021 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1081 cgccgccggg atcactctcg gcatggacga gctgtacaag taaggtaccg gatccgaatt
     1141 cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc
     1201 cggctgctaa caa
//
