LOCUS       sequence-99670-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene targeting construct for human NANOS3 locus, introducing a 
            P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette 
            that can be excised..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Irie N, Weinberger L, Tang WW, Kobayashi T, Viukov S, Manor YS, 
            Dietmann S, Hanna JH, Surani MA
  TITLE     SOX17 is a critical specifier of human primordial germ cell fate.
  JOURNAL   Cell. 2015 Jan 15;160(1-2):253-68. doi: 10.1016/j.cell.2014.12.013. 
            Epub 2014 Dec 24.
  PUBMED    25543152
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2015-01-15"; volume: "160(1-2):253-68. doi"; pages: " 
            10.1016/j.cell.2014.12.013. Epub 2014 Dec 24"
COMMENT     Sequence Label: Human NANOS3-P2A-mCherry knock-in targeting
            co...
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
       61 actccttgat gatggccatg ttatcctcct cgcccttgct caccatggta agcttcagct
      121 gaagcttgca tgcgggcccg gggttggact cgacgtctcc cgcaagctta agaaggtcaa
      181 aattcaaagt ttgcttacca gaaccgtcga ctctagagtc gcgaccgctg taggtggaca
      241 tggagggaga gcaggagggc gaaggctcag acttcccggc acctctgaaa cctagtggag
      301 ggggagggag agaaatgctt aatcacaggc gatgactcaa cgctccccaa gttgcccacg
      361 gactcggcct ccgggcctgc tggacctctg gcgccaccca ggggctgctc cggagacagc
      421 gaccccgtgg cgtctctggc tccgccgggc ggccccgctg ggacccagag cccgagacaa
      481 agcacaggag ttccgattct gcgactgtgg tgccctctcg tggccgccgt gctctggggt
      541 ccccggatct gttactccca aaagttgttc atcagaaagg ctggccgcag gacaccatct
      601 tccctatgtc cttttggcct ccagcgtctc ccagtgagac agcatcaaac ctcacaggaa
      661 acactatctt ctcctggagg cagtctttca tgctttcttt tttttgtgag acggagtctc
      721 actctgtcgc ccaggctgga gtgcagtgac gtgacctcag ctcactgcga cctccgcctc
      781 ccgggttcta gcgattctcc tgtctcagcc c
//
