LOCUS       sequence-99683-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloned genomic fragment of murine Nanog locus used as probe for 
            Southern Blot analysis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Maza I, Caspi I, Zviran A, Chomsky E, Rais Y, Viukov S, Geula S, 
            Buenrostro JD, Weinberger L, Krupalnik V, Hanna S, Zerbib M, Dutton 
            JR, Greenleaf WJ, Massarwa R, Novershtern N, Hanna JH
  TITLE     Transient acquisition of pluripotency during somatic cell 
            transdifferentiation with iPSC reprogramming factors.
  JOURNAL   Nat Biotechnol. 2015 Jun 22. doi: 10.1038/nbt.3270.
  PUBMED    26098448
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Jun 22. doi: 10.1038/nbt.3270."
COMMENT     Sequence Label: Mouse Nanog locus 3' probe
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(811..830)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(812..830)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(837..854)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(837..853)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctagtaacgg ccgccagtgt gctggaattc gcccttggac agttgccaga cagaggaggg
       61 agaggagccc gagcagggga aaaaggagga tttgttaaat aaagaagtca gctggggtcc
      121 tgcaacagct tgggactcag atgtcagttg actaaggcat ttgaataggt cccttggtgg
      181 gagcctgaag caaaaagcct ctgaaagctg cagctgggga ggcctcttga actagcccca
      241 ctcctgggtg attggtggaa tctcttccac cctaaaaagg gtaaaatcaa agctcggatt
      301 ctcacaccaa ggactgagtg ttgttcagta gtagagcact tacccagggt ctaagagtcc
      361 ctggatttga tccccaagac cacacatttt agaatcttat caacgaaacc tctaagagtt
      421 gaagcaaagt ggtctctcag taacggtcgt cagggtttgt ggaggtgatg atgtcccatc
      481 tctgacacag cagccatccc cactgatgcc aaaggacaga aagcagaaga ggagaattct
      541 aggtggaatt agtctgggct ttgcaaggga atgtgtccta gcctctgtgg cacaatgagg
      601 gacaattttt ctcactgcca aaacagccct ctccttagct gaggagcagc tacagaacct
      661 acttgggctg ggctgggtac ccctgtgagt ggtcaggagt taaagatgtg attgacaggt
      721 atggctattt acgcacggca gaagggcgaa ttctgcagat atccatcaca ctggcggccg
      781 ctcgagcatg catctagagg gcccaattcg ccctatagtg agtcgtatta caattcactg
      841 gccgtcgttt tacaacgtcg tgactg
//
