LOCUS       sequence-99690-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged mouse TUT4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Liu X, Zheng Q, Vrettos N, Maragkakis M, Alexiou P, Gregory BD, 
            Mourelatos Z
  TITLE     A MicroRNA Precursor Surveillance System in Quality Control of 
            MicroRNA Synthesis.
  JOURNAL   Mol Cell. 2014 Aug 27. pii: S1097-2765(14)00610-8. doi: 
            10.1016/j.molcel.2014.07.017.
  PUBMED    25175028
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 Aug 27. pii: S1097-2765(14)00610-8. doi: 
            10.1016/j.molcel.2014.07.017."
COMMENT     Sequence Label: pCI-FLAG-mTUT4
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             43..66
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagtcttaat acgactcact ataggctagc ctcgagacca tggactacaa agacgatgac
       61 gacaaggaag agcccaagac ttctaaaaac gaaaatcatg aaccaaagaa gaatattatt
      121 tgtgaagaaa gcaaagcagt taaaattata tctaatcaaa ccttgaagcc tagaaatgat
      181 aagtcagaaa ttgggaccag ctctctgaat agaaatagta gtaaaaaaac caagcaaaat
      241 gatatttgta tagaaaaaac agaagctaaa tcatgtaaag taaacgctgc cagtgttcca
      301 ggccctaaag atttggggtt agtacatcga gatcaaagtc attgcaaaat gaaaaagtta
      361 ccaaattcac ccatgaaagc ccagaaggga tcttcacaga caaaattaga aaagacacct
      421 agtttacaga caaaagcaga aaaagtacca aagtctccaa atttaccagt aaaagcagaa
      481 aaagcgccct gtacaacagc agaagcaaca acagaaaaag cacttaattc acagagaaaa
      541 gaagaaaaca cacccacttc tcagatgaaa ttacagaaga cacccagatc acctttagag
      601 ccagaaaatg tgcccagttt actattgaaa gaaaatgtga aacagacaga atcacaacag
      661 actgggaaga aacttacaag ctcctttgtt tctatggata aaaggaagag tgaagctcta
      721 cagggagaaa agtcagctct ggaaaactca tcactatctc agaagcagca gactcagaca
      781 gataatattg ctgattctga tgacagtgct tca
//
