LOCUS       sequence-99697-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene targeting construct for human NANOS3 locus, introducing a 
            P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette 
            that can be excised..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Irie N, Weinberger L, Tang WW, Kobayashi T, Viukov S, Manor YS, 
            Dietmann S, Hanna JH, Surani MA
  TITLE     SOX17 is a critical specifier of human primordial germ cell fate.
  JOURNAL   Cell. 2015 Jan 15;160(1-2):253-68. doi: 10.1016/j.cell.2014.12.013. 
            Epub 2014 Dec 24.
  PUBMED    25543152
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2015-01-15"; volume: "160(1-2):253-68. doi"; pages: " 
            10.1016/j.cell.2014.12.013. Epub 2014 Dec 24"
COMMENT     Sequence Label: Human NANOS3-P2A-mCherry knock-in targeting
            co...
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(120..137)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    126..350
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(393..426)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    444..477
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
       61 ctatcgcctt cttgacgagt tcttctgagg ggatcaattc tctagagctc gctgatcagc
      121 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
      181 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
      241 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
      301 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
      361 ggaaagaacc agctgggggc gcgcccctcg aggaagttcc tatactttct agagaatagg
      421 aacttcggta ccctagaaag cttataactt cgtataatgt atgctatacg aagttataga
      481 tctcctagag ttaacgaggc tgcctacacc tgggcaaggg caccggggct cggctggatt
      541 tccaggaaga cccaccctat gacgactgcc cccactccca gaccctcccc ccagcctggg
      601 attgagccct ggggatgacc cggggttggc aagggaagag ctgaaatcgc cctgtccttg
      661 gggaccccac cctttgtgcc atctgccgtt tccctagtgc tgggcatcca gtcagccctc
      721 aataaatgct tatgaatgga tcaacaacag acaagccctg gtcttgggac aaggtgtagt
      781 gtgaaccttg ctgggagtcc tgtacctaga tctccc
//
