LOCUS       sequence-99715-e         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged mouse RRP6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Liu X, Zheng Q, Vrettos N, Maragkakis M, Alexiou P, Gregory BD, 
            Mourelatos Z
  TITLE     A MicroRNA Precursor Surveillance System in Quality Control of 
            MicroRNA Synthesis.
  JOURNAL   Mol Cell. 2014 Aug 27. pii: S1097-2765(14)00610-8. doi: 
            10.1016/j.molcel.2014.07.017.
  PUBMED    25175028
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 Aug 27. pii: S1097-2765(14)00610-8. doi: 
            10.1016/j.molcel.2014.07.017."
COMMENT     Sequence Label: pCI-FLAG-mRRP6
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             49..72
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagtacttaa tacgactcac tataggctag cctcgagaat tcaccatgga ctacaaagac
       61 gatgacgaca aggcgcctcc cagtccccgg gagcatcagt ccgcgccggc gaccagtgcg
      121 accaagcctg acgcggagat ggtactgcct ggcttccccg atgcagacag cttcgtaaag
      181 tttgcacttg ggtcggtagt ggcagttacc aaggcatccg ggggcctgcc acagttcggt
      241 gacgagtatg atttttacag aagtttccct gccttccagg cattctgtga gacacaagga
      301 gacaggttac tgcagtgcat gagtcgggta atgcagtacc atggctgtcg cagcaacatc
      361 aaagaccgaa gtaaagtgac tgaattggag gacaagtttg atttattagt cgataccaat
      421 gacgtgatat tggagagagt gggcatgtta ctggatgaag cctccggtgt gaacaagcat
      481 cagcagcctg tccttcctgc agggctgcag gtccccaaaa caatagtatc cagctggaat
      541 cggaaggcag gagagtatgg caaaaaggca aaatcggaga ctttccgact gctgcatgca
      601 aaaaacatcg tgcgacctca gctcaggttt cgagagaaga tcgacaattc taacacacca
      661 tttctcccga agatctttgt caaacccaat gcccggaagc cactccctct ggctctctc
//
