LOCUS       sequence-99717-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged catalytic mutant of TUT4 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Liu X, Zheng Q, Vrettos N, Maragkakis M, Alexiou P, Gregory BD, 
            Mourelatos Z
  TITLE     A MicroRNA Precursor Surveillance System in Quality Control of 
            MicroRNA Synthesis.
  JOURNAL   Mol Cell. 2014 Aug 27. pii: S1097-2765(14)00610-8. doi: 
            10.1016/j.molcel.2014.07.017.
  PUBMED    25175028
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 Aug 27. pii: S1097-2765(14)00610-8. doi: 
            10.1016/j.molcel.2014.07.017."
COMMENT     Sequence Label: pCI-FLAG-mTUT4(D1028A)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             57..80
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgctcttagg ctagagtact taatacgact cactataggc tagcctcgag accatggact
       61 acaaagacga tgacgacaag gaagagccca agacttctaa aaacgaaaat catgaaccaa
      121 agaagaatat tatttgtgaa gaaagcaaag cagttaaaat tatatctaat caaaccttga
      181 agcctagaaa tgataagtca gaaattggga ccagctctct gaatagaaat agtagtaaaa
      241 aaaccaagca aaatgatatt tgtatagaaa aaacagaagc taaatcatgt aaagtaaacg
      301 ctgccagtgt tccaggccct aaagatttgg ggttagtaca tcgagatcaa agtcattgca
      361 aaatgaaaaa gttaccaaat tcacccatga aagcccagaa gggatcttca cagacaaaat
      421 tagaaaagac acctagttta cagacaaaag cagaaaaagt accaaagtct ccaaatttac
      481 cagtaaaagc agaaaaagcg ccctgtacaa cagcagaagc aacaacagaa aaagcactta
      541 attcacagag aaaagaagaa aacacaccca cttctcagat gaaattacag aagacaccca
      601 gatcaccttt agagccagaa aatgtgccca gtttactatt gaaagaaaat gtgaaacaga
      661 cagaatcaca acagactggg aagaaactta caagctcctt tgtttctatg gataaaagga
      721 agagtgaagc tctacaggga gaaaagtcag ctctggaaaa ctcatcacta tctcagaagc
      781 agcagactca gacagataat attgctgatt ctgatgacag tgcttcaggg attgaagata
      841 cagcagatga
//
