LOCUS       sequence-99719-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloned genomic fragment of murine Nanog locus used as probe for 
            Southern Blot analysis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Maza I, Caspi I, Zviran A, Chomsky E, Rais Y, Viukov S, Geula S, 
            Buenrostro JD, Weinberger L, Krupalnik V, Hanna S, Zerbib M, Dutton 
            JR, Greenleaf WJ, Massarwa R, Novershtern N, Hanna JH
  TITLE     Transient acquisition of pluripotency during somatic cell 
            transdifferentiation with iPSC reprogramming factors.
  JOURNAL   Nat Biotechnol. 2015 Jun 22. doi: 10.1038/nbt.3270.
  PUBMED    26098448
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Jun 22. doi: 10.1038/nbt.3270."
COMMENT     Sequence Label: Mouse Nanog locus 3' probe
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(838..854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(838..854)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(851..873)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    862..878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cctctagatg catgctcgag cggccgccag tgtgatggat atctgcagaa ttcgcccttc
       61 tgccgtgcgt aaatagccat acctgtcaat cacatcttta actcctgacc actcacaggg
      121 gtacccagcc cagcccaagt aggttctgta gctgctcctc agctaaggag agggctgttt
      181 tggcagtgag aaaaattgtc cctcattgtg ccacagaggc taggacacat tcccttgcaa
      241 agcccagact aattccacct agaattctcc tcttctgctt tctgtccttt ggcatcagtg
      301 gggatggctg ctgtgtcaga gatgggacat catcacctcc acaaaccctg acgaccgtta
      361 ctgagagacc actttgcttc aactcttaga ggtttcgttg ataagattct aaaatgtgtg
      421 gtcttgggga tcaaatccag ggactcttag accctgggta agtgctctac tactgaacaa
      481 cactcagtcc ttggtgtgag aatccgagct ttgattttac cctttttagg gtggaagaga
      541 ttccaccaat cacccaggag tggggctagt tcaagaggcc tccccagctg cagctttcag
      601 aggctttttg cttcaggctc ccaccaaggg acctattcaa atgccttagt caactgacat
      661 ctgagtccca agctgttgca ggaccccagc tgacttcttt atttaacaaa tcctcctttt
      721 tcccctgctc gggctcctct ccctcctctg tctggcaact gtccaagggc gaattccagc
      781 acactggcgg ccgttactag tggatccgag ctcggtacca agcttggcgt aatcatggtc
      841 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      901 aa
//
