LOCUS       sequence-99734-e        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Firefly luciferase (Promega) driven by RNR1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB783
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1040..1060)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1040..1058)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggatagaatg gcgccgggcc tttctttatg tttttgacgt cttccatgtt aattaagatg
       61 ttaatatatc aacaaataaa gtgttgttcg ttggtgtctc tcttgcgttg aaaaggatga
      121 aaaggatgag atatgcttca attatggtag agtaattttc caacaatgaa atattagctc
      181 ctttatataa tttttattca atggtactga cttaacaaag gcaacaagat aaactctaaa
      241 cttgttattc taaaacgtga gctgcagact ttgttggagg tttctatcaa acacgaaact
      301 acctacgcca acctattctt tctacactat atgaaaaaaa tttccatatc gtttttacgc
      361 gtcctctatt caacacccaa taagattctg caaggatttc tgtgtcctga ggatcaaaat
      421 gacgcgttta cttagaattt caaaaaaaga agcaattaaa gtaacagagg atgaacttac
      481 ccgacactgt ttacgcgttt tttcttttta aatttgattt gggtgccata taatgggata
      541 aaacgcgtaa acaagctcgt tccagggcta cgaaatattt ctacccagaa gtggtcgtaa
      601 aataattaac gatagtaaac aaaggccata gaaagttcag cattgacttg gtaacgagat
      661 tttccccaat ggaattcctt tgtatgaaag attctcctga agagtaattc tgccaatcag
      721 tacatttcta tggtaacgcc tttgtttacg atatccaaat gcggcaatcc ttctctgttg
      781 tggtgatgtc gtgataccat aaagtggtat tataattttg gatgtaagga gatcaaagat
      841 cttgaaatca ataattattc ttatggctac tcaaaatcaa tacatgagaa gcactatttt
      901 tagaccatgt cctacttcgt tatttaatgg atatatatat atttatatat ttatatacgt
      961 taatgtctca tgtgactgag ctgcagatct actatacaca gtgacggccg ccaccgcggt
     1021 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatgg
//
