LOCUS       sequence-99742-e         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express HA-tagged wild-type Ago2 in mammalian cells in a 
            Dox-inducible manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Liu X, Jin DY, McManus MT, Mourelatos Z
  TITLE     Precursor microRNA-programmed silencing complex assembly pathways in
            mammals.
  JOURNAL   Mol Cell. 2012 May 25;46(4):507-17. doi: 
            10.1016/j.molcel.2012.03.010. Epub 2012 Apr 12.
  PUBMED    22503104
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.03.010"; journalName: "Mol Cell"; date: 
            "2012-05-25- 25"; volume: "46"; issue: "4"; pages: "507-17"
COMMENT     Sequence Label: pSLIK-Hygro-Myc-hAgo2
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(47..65,66..88,89..107,108..130,131..149,150..172,
                     173..191,192..214,215..233,234..256,257..275,276..298,
                     299..317)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        350..388
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     385..409
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             496..525
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcaggcttta aaggaaccaa ttcagtcgac tggatccttt accacgtccc tatcagtgat
       61 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      121 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      181 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
      241 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
      301 cctatcagtg atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt
      361 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      421 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc
      481 cgaactagta ccatggagca aaagctcatc tcagaggaag atctggaatt caggatcctc
      541 atgtactcgg gagccggccc cgcacttgca cctcctgcgc cgccgccccc catccaagga
      601 tatgccttca agcctccacc tagacccgac tttgggacct ccgggagaac aatcaaatta
      661 caggccaatt tcttcgaaat g
//
