LOCUS       sequence-99744-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express HA-tagged wild-type Ago2 in mammalian cells in a 
            Dox-inducible manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Liu X, Jin DY, McManus MT, Mourelatos Z
  TITLE     Precursor microRNA-programmed silencing complex assembly pathways in
            mammals.
  JOURNAL   Mol Cell. 2012 May 25;46(4):507-17. doi: 
            10.1016/j.molcel.2012.03.010. Epub 2012 Apr 12.
  PUBMED    22503104
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.03.010"; journalName: "Mol Cell"; date: 
            "2012-05-25- 25"; volume: "46"; issue: "4"; pages: "507-17"
COMMENT     Sequence Label: pSLIK-Hygro-Myc-hAgo2
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    146..170
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atcaggacgc tcgctgcgcc cttcgtctga cgtggcagcg ctcgccgctg aggagggggg
       61 cgcccgcggg aggcgccaaa acccggcgcg gaggccagat cttgggtggg ttaattaacc
      121 atctttatgg ctcgagatgt tatcaaccac tttgtacaag aaagctgggt ctagatatct
      181 cgagtgcggc cgctcaagca aagtacatgg tgcgcagagt gtcttggtga acctggaccg
      241 ccttggccag tgcttggtgg tctcgcccgt tactctgccc agaggtatgg cttccttcag
      301 cactgtcatg ttccttatcc accaggtggt acctggcccg gaaggccacc aggtgagcgt
      361 agtatgctgg cgctgggatg gacacggagc gtgtgcagcg cacgtaggtg tgacacagct
      421 ggtaggttag gatctgcagc tcatcagagg agaaacgatt gtcgtcccag aggacgtgat
      481 agtgcgaagg cctgcttgtc ccctggatgc cagcgtgact acacaggtag aagtcgaact
      541 cggtggggtg ggtgattttc gtgtccacag tcgtgcctgc tggaatgttt ccacttttcc
      601 caacccgctc gttcttgtca gtgcagaaga gccgggtgtg gtgcctcttc tgcaccacga
      661 tgaaggtgat cccgggctgg tagtcttttt ctagcttgat acaggcctca cggatggcca
      721 gcaactcgtg gtggag
//
