LOCUS       sequence-99752-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer vector driving expression of 
            human TGF-beta3 and RFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Brunger JM, Huynh NP, Guenther CM, Perez-Pinera P, Moutos FT, 
            Sanchez-Adams J, Gersbach CA, Guilak F
  TITLE     Scaffold-mediated lentiviral transduction for functional tissue 
            engineering of cartilage.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E798-806. doi: 
            10.1073/pnas.1321744111. Epub 2014 Feb 18.
  PUBMED    24550481
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1321744111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-03-4- 4"; volume: "111"; issue: "9"; pages: "E798-806"
COMMENT     Sequence Label: pLVE-hTGFB3-IRES-Red
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    52..169
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtgaggaatt tcgacattta aatttaatta atctcgacgg tatcggttaa cttttaaaag
       61 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
      121 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatcacga
      181 gactagcctc gaggtttaaa ctacgggatc cgtactttta tctgtcccct ccaccccaca
      241 gtggggcaag cttgaattct ctagaaatat tctcgaggcc gccaccatga agatgcactt
      301 gcaaagggct ctggtggtcc tggccctgct gaactttgcc acggtcagcc tctctctgtc
      361 cacttgcacc accttggact tcggccacat caagaagaag agggtggaag ccattagggg
      421 acagatcttg agcaagctca ggctcaccag cccccctgag ccaacggtga tgacccacgt
      481 cccctatcag gtcctggccc tttacaacag cacccgggag ctgctggagg agatgcatgg
      541 ggagagggag gaaggctgca cccaggaaaa caccgagtcg gaatactatg ccaaagaaat
      601 ccataaattc gacatgatcc aggggctggc ggagcacaac gaactggctg tctgccctaa
      661 aggaattacc tccaaggttt tccgcttcaa tgtgtcctca gtggagaaaa atagaaccaa
      721 cctattccga gcagaattcc gggtcttgcg ggtgcccaa
//
