LOCUS       sequence-99753-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector driving expression of eGFP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Brunger JM, Huynh NP, Guenther CM, Perez-Pinera P, Moutos FT, 
            Sanchez-Adams J, Gersbach CA, Guilak F
  TITLE     Scaffold-mediated lentiviral transduction for functional tissue 
            engineering of cartilage.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E798-806. doi: 
            10.1073/pnas.1321744111. Epub 2014 Feb 18.
  PUBMED    24550481
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1321744111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-03-4- 4"; volume: "111"; issue: "9"; pages: "E798-806"
COMMENT     Sequence Label: pLVE-eGFP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          400..603
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     560..580
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    660..777
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      823..832
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
       61 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
      121 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
      181 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
      241 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
      301 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
      361 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
      421 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
      481 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
      541 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
      601 caggtgtcgt gaggaatttc gacatttaaa tttaattaat ctcgacggta tcggttaact
      661 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
      721 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc
      781 gatcacgaga ctagcctcga ggtttaaact acgggatctg ccgccaccat ggtgagcaag
      841 ggcgaggagc
//
